Elespuru R K, Lijinsky W
Cancer Res. 1976 Nov;36(11 Pt 1):4099-101.
Ten cyclic nitrosamines were tested for mutagenicity in Escherichia coli after incubation in vitro with 9000 X g microsomal supernatants prepared from rat liver, and the results were compared with carcinogenicity data from the same species. None of the compounds was mutagenic in the absence of microsomes. Seven carcinogenic compounds, nitrosopyrrolidine, nitrosopiperidine, nitrosohexamethyleneimine, nitrosoheptamethyleneimine, nitrosomorpholine, dinitrosopiperazine, and dinitrosohomopiperzine, were mutagenic after microsomal activation. One compound, nitrosohepamethyleneimine, was toxic to the bacteria. Two noncarcinogens, 1-nitrosopiperazine and 1-methyl-4-nitrosopiperazine, and 1 strong carcinogen, 2,6-dimethyldinitrosopiperazine, were not mutagenic with or without microsomal incubation. The liver microsome preparation activated equally well those compounds that are liver carcinogens in Sprague-Dawley rats, and compounds for which the liver is not a target organ.
十种环状亚硝胺在体外与从大鼠肝脏制备的9000×g微粒体上清液孵育后，在大肠杆菌中进行了致突变性测试，并将结果与来自同一物种的致癌性数据进行了比较。在没有微粒体的情况下，没有一种化合物具有致突变性。七种致癌化合物，亚硝基吡咯烷、亚硝基哌啶、亚硝基六亚甲基亚胺、亚硝基七亚甲基亚胺、亚硝基吗啉、二亚硝基哌嗪和二亚硝基高哌嗪，在微粒体激活后具有致突变性。一种化合物，亚硝基庚亚甲基亚胺，对细菌有毒。两种非致癌物，1-亚硝基哌嗪和1-甲基-4-亚硝基哌嗪，以及一种强致癌物，2,6-二甲基二亚硝基哌嗪，无论有无微粒体孵育都不具有致突变性。肝脏微粒体制剂对那些在斯普拉格-道利大鼠中为肝脏致癌物的化合物以及肝脏不是靶器官的化合物的激活效果相同。
