Stoltz D R, Sen N P
J Natl Cancer Inst. 1977 Feb;58(2):393-4. doi: 10.1093/jnci/58.2.393.
The mutagenicity of five cyclic N-nitrosamines was studied with the use of Salmonella typhimurium TA1535 in vitro with and without microsomal activation. The carcinogens nitrosopiperidine and nitrosopyrrolidine required metabolic activation before manifesting mutagenic activity. Nitrosoproline and nitrosohydroxyproline, noncarcinogens, were not mutagenic. Nitroso-3-pyrrolidinol was mutagenic in the absence of microsomes, thereby suggesting a role of hydroxylation in the metabolic activation of nitrosopyrrolidine to an ultimate carcinogenic species.
利用鼠伤寒沙门氏菌TA1535在体外研究了五种环状N-亚硝胺的诱变性，实验分别在有和没有微粒体激活的情况下进行。致癌物亚硝基哌啶和亚硝基吡咯烷在表现出诱变活性之前需要代谢激活。非致癌物亚硝基脯氨酸和亚硝基羟脯氨酸没有诱变性。亚硝基-3-吡咯烷醇在没有微粒体的情况下具有诱变性，因此表明羟基化在亚硝基吡咯烷代谢激活为最终致癌物质的过程中发挥了作用。
