Dandeu J P, Lux M
Immunol Commun. 1978;7(4):393-415. doi: 10.3109/08820137809047625.
Two proteins, FI and FVII, have been purified from a whole worm extract of adult Ascaris suum, using ammonium sulfate precipitation, Sephadex gel filtration and DEAE cellulose chromatography. Both of them provoke reagin formation in mice as tested in presensitized animals with an original immediate hypersensitivity reaction test. They are two chemically and physically different protein molecules showing no cross reaction with a rabbit serum anti-crude extract but bearing common allergenic epitopes as proved when tested in mice.
已利用硫酸铵沉淀、葡聚糖凝胶过滤和二乙氨基乙基纤维素色谱法，从成年猪蛔虫的全虫提取物中纯化出两种蛋白质，即FI和FVII。在预先致敏的动物中通过原始速发型超敏反应试验检测发现，这两种蛋白质均可在小鼠体内引发反应素形成。它们是两种化学和物理性质不同的蛋白质分子，与兔抗粗提取物血清无交叉反应，但在小鼠试验中证明具有共同的过敏原表位。
