5-氟尿嘧啶、维拉帕米和热疗对人胃腺癌细胞（AGS）的体外抗肿瘤活性。

Antitumour activity of 5-fluorouracil, verapamil and hyperthermia against human gastric adenocarcinoma cell (AGS) in vitro.

作者信息

Shchepotin I B, Shabahang M, Nauta R J, Buras R R, Brenner R V, Evans S R

机构信息

Lombardi Cancer Center, Department of Surgery, Georgetown University, Washington, DC.

出版信息

Surg Oncol. 1994 Oct;3(5):287-94. doi: 10.1016/0960-7404(94)90031-0.

DOI:10.1016/0960-7404(94)90031-0

PMID:7889222

Abstract

The purpose of this study was to assess the efficacy of verapamil (20 microM) and hyperthermia (42 degrees C) as modifiers of 5-fluorouracil (5-FU), used at different concentrations, in inhibiting the growth of gastric adenocarcinoma cells. Combined verapamil and hyperthermia treatment showed a significant decrease in cell count when compared to control (72.2%), hyperthermia alone (68.4%), or verapamil alone (65%). At a high concentration of 5-FU (50 micrograms/ml), verapamil and hyperthermia had an additive growth inhibitory effect over a 4-day period when compared to control. A combination of 5-FU at low concentration (0.5 microgram/ml) with verapamil significantly suppressed growth by 31.2% in comparison to control--with this effect being independent of the duration of treatment. The modalities analysed in this study require further investigation and have potential for clinical applicability to gastric cancer therapy in the future.

摘要

本研究的目的是评估维拉帕米（20微摩尔）和热疗（42摄氏度）作为不同浓度5-氟尿嘧啶（5-FU）的修饰剂，在抑制胃腺癌细胞生长方面的效果。与对照组（72.2%）、单独热疗组（68.4%）或单独维拉帕米组（65%）相比，维拉帕米与热疗联合治疗显示细胞计数显著下降。在高浓度5-FU（50微克/毫升）时，与对照组相比，维拉帕米和热疗在4天时间内具有相加的生长抑制作用。低浓度5-FU（0.5微克/毫升）与维拉帕米联合使用时，与对照组相比，生长显著抑制31.2%，且这种作用与治疗持续时间无关。本研究中分析的这些方式需要进一步研究，并且未来在胃癌治疗中具有临床应用潜力。