对来自西班牙巴塞罗那城市空气中颗粒物的遗传毒性成分进行生物测定导向的化学分析。

Bioassay-directed chemical analysis of genotoxic components in urban airborne particulate matter from Barcelona (Spain).

作者信息

Casellas M, Fernandez P, Bayona J M, Solanas A M

机构信息

Microbiology Department, School of Biology, University of Barcelona, Spain.

出版信息

Chemosphere. 1995 Feb;30(4):725-40. doi: 10.1016/0045-6535(94)00438-z.

DOI:10.1016/0045-6535(94)00438-z

PMID:7889349

Abstract

Organic extracts of airborne particulate matter, collected in the city of Barcelona, were subjected to three-level, bioassay-directed, chemical fractionation, including gel permeation chromatography (GPC) and normal-phase (NP) and reversed-phase (RP) liquid chromatography (LC). The chemical characterization, directed by the Salmonella microsome mutagenicity assay (TA98, TA98NR and TA98/1,8DNP6 +/- S9), was carried out by capillary GC (CGC) coupled to selective detection systems, and by GC-MS techniques. The results obtained with the nitroreductase deficient strains show the important contribution of nitroaromatic compounds. Detailed chemical analysis of the mutagenic fractions led to the identification of 82 aromatic compounds and revealed the large contribution of chemical classes that are more polar than polycyclic aromatic hydrocarbons such as aromatic ketones, quinones and aldehydes.

摘要

在巴塞罗那市收集的空气传播颗粒物的有机提取物，经过了三级生物测定导向的化学分级分离，包括凝胶渗透色谱法（GPC）以及正相（NP）和反相（RP）液相色谱法（LC）。在沙门氏菌微粒体诱变性试验（TA98、TA98NR和TA98/1,8DNP6 +/- S9）的指导下，通过与选择性检测系统联用的毛细管气相色谱法（CGC）以及气相色谱-质谱联用（GC-MS）技术进行化学表征。用硝基还原酶缺陷菌株获得的结果表明了硝基芳香化合物的重要贡献。对诱变级分的详细化学分析导致鉴定出82种芳香族化合物，并揭示了比多环芳烃极性更强的化学类别（如芳香酮、醌和醛）的巨大贡献。