Matsukura N, Onda M, Tokunaga A, Matsuda N, Yamashita K
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Cancer. 1995 Mar 15;75(6 Suppl):1472-7. doi: 10.1002/1097-0142(19950315)75:6+<1472::aid-cncr2820751515>3.0.co;2-8.
Subjects with atrophic body gastritis have a high prevalence of Helicobacter pylori seropositivity and a low prevalence of H. pylori infection. Disappearance of the organism appears to correlate with the development of intestinal metaplasia. To investigate this point, intestinal metaplasia was biochemically subclassified into complete and incomplete types by the Tes-Tape method, and tissue IgA and IgG antibodies against H. pylori were measured by enzyme-linked immunosorbent assay (ELISA).
Twenty-five stomachs resected for gastric cancer were examined using the Tes-Tape method. Serum H. pylori IgA and IgG antibodies and tissue IgA and IgG antibodies against H. pylori and tissue secretory IgA (sc-IgA) were examined in areas of intestinal metaplasia, nonmetaplastic gastric mucosa, and gastric carcinoma by ELISA:
Tissue H. pylori IgA antibody was positive in 6 of 19 (32%) specimens taken from complete and 2 of 7 (29%) incomplete types of intestinal metaplasia and was positive in 6 of 14 (43%) nonmetaplastic gastric mucosa from the antrum and 14 of 23 (61%) from the body. Duodenal mucosa and cancer tissue were positive for tissue IgA antibody in 1 of 6 (17%) and 0 of 17 (0%), respectively. Tissue H. pylori IgG antibody was negative in all the tissues examined. sc-IgA in the areas of intestinal metaplasia was 120 +/- 65 (mean +/- standard error; ng/mg wet weight) and in the nonmetaplastic gastric mucosa was 113 +/- 72, showing no difference. Positivity and negativity of serum IgA and IgG antibodies against H. pylori coincided with presence or absence of tissue IgA antibody in nonmetaplastic gastric mucosa in 15 of 19 (79%) and 16 of 19 (84%) patients examined, respectively.
Positivity rates of tissue IgA antibody against H. pylori were lower in the mucosa of intestinal metaplasia than in nonmetaplastic gastric mucosa and were negative in carcinoma. No significant difference in levels of sc-IgA between intestinal metaplasia and non-metaplastic gastric mucosa was found.
萎缩性胃体胃炎患者幽门螺杆菌血清阳性率高，而幽门螺杆菌感染率低。该菌的消失似乎与肠化生的发展相关。为研究这一点，采用泰斯试纸法将肠化生进行生化亚分类，分为完全型和不完全型，并通过酶联免疫吸附测定（ELISA）检测组织中抗幽门螺杆菌的IgA和IgG抗体。
对25例因胃癌切除的胃标本采用泰斯试纸法进行检测。通过ELISA检测肠化生区域、非化生胃黏膜和胃癌组织中的血清幽门螺杆菌IgA和IgG抗体以及组织中抗幽门螺杆菌的IgA和IgG抗体和组织分泌型IgA（sc-IgA）：
在取自完全型肠化生的19个标本中有6个（32%）、不完全型肠化生的7个标本中有2个（29%）组织幽门螺杆菌IgA抗体呈阳性；在取自胃窦的14个非化生胃黏膜标本中有6个（43%）、取自胃体的23个标本中有14个（61%）呈阳性。十二指肠黏膜和癌组织中组织IgA抗体阳性率分别为6个中的1个（17%）和17个中的0个（0%）。在所检测的所有组织中，组织幽门螺杆菌IgG抗体均为阴性。肠化生区域的sc-IgA为120±65（平均值±标准误；ng/湿重mg），非化生胃黏膜为113±72，无差异。在接受检测的19例患者中，血清抗幽门螺杆菌IgA和IgG抗体的阳性和阴性分别与非化生胃黏膜中组织IgA抗体的存在或不存在相符，分别为19例中的15例（79%）和19例中的16例（84%）。
肠化生黏膜中组织抗幽门螺杆菌IgA抗体阳性率低于非化生胃黏膜，在癌组织中为阴性。未发现肠化生与非化生胃黏膜之间sc-IgA水平有显著差异。
