Laguens Graciela, Coronato Silvia, Laguens Rubén, Portiansky Enrique, Di Girolamo Vanda
Departamento de Patología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 65 No. 309 (1 y 2), La Plata 1900, Argentina.
Immunol Lett. 2002 Dec 3;84(3):159-62. doi: 10.1016/s0165-2478(02)00172-4.
Dendritic cells (DCs) are bone-marrow derived 'professional' antigen presenting cells (APC). They are considered as the most potent APC able to induce primary immune responses. DC efficiently capture and process proteic and non-proteic antigens. They are widely distributed throughout the body and occupy sentinel positions such as epithelia. Establishment of an immune response against cancer may depend of the capacity of DCs to transfer (to capture, to process and to present) tumor antigens into regional lymph nodes where they can induce a specific response leading to tumor rejection. Because host 'professional' DCs are one of the most important elements in the induction of specific anti-tumor responses and lymph nodes are the places where the immune response takes place, we investigated the densities of DCs within regional metastasis-free lymph nodes from 47 patients with different malignant epithelial tumors as comparing with lymph nodes from 11 patients without malignancies using an immunohistochemistry method with anti-S100 protein, CD86 and CD1a antibodies. By means of morphometric analysis, we observed that S100+ and CD1a+ DCs densities in regional lymph nodes from cancer patients were significatively decreased as compared with control lymph nodes (P<0.0001 and 0.003, respectively). S100+ DCs and CD86+ DCs densities in lymph nodes draining cancer were similar. Taken together, these data indicated that lymph nodes draining cancer had significantly less CD1a+ DCs than S100+ and possibly CD86+ DCs. These findings may represent another mechanism by which tumors evade the immune recognition.
树突状细胞（DCs）是源自骨髓的“专职”抗原呈递细胞（APC）。它们被认为是能够诱导初次免疫反应的最有效的APC。DC能有效地捕获和处理蛋白质和非蛋白质抗原。它们广泛分布于全身，并占据诸如上皮等哨兵位置。针对癌症建立免疫反应可能取决于DC将肿瘤抗原转移（捕获、处理和呈递）至区域淋巴结的能力，在区域淋巴结中它们可诱导导致肿瘤排斥的特异性反应。由于宿主“专职”DC是诱导特异性抗肿瘤反应的最重要因素之一，且淋巴结是免疫反应发生的场所，我们采用抗S100蛋白、CD86和CD1a抗体的免疫组织化学方法，研究了47例不同恶性上皮肿瘤患者区域无转移淋巴结内DC的密度，并与11例无恶性肿瘤患者的淋巴结进行比较。通过形态计量分析，我们观察到癌症患者区域淋巴结中S100 +和CD1a + DC的密度与对照淋巴结相比显著降低（分别为P <0.0001和0.003）。引流癌症的淋巴结中S100 + DC和CD86 + DC的密度相似。综上所述，这些数据表明引流癌症的淋巴结中CD1a + DC明显少于S100 + DC，可能也少于CD86 + DC。这些发现可能代表了肿瘤逃避免疫识别的另一种机制。
