van Kreel B K
Department of Clinical Chemistry, University Hospital Maastricht, The Netherlands.
Clin Chim Acta. 1994 Dec 16;231(2):117-28. doi: 10.1016/0009-8981(94)90196-1.
A sensitive bromide determination has been developed that consists of the following steps: isolation (purification) of bromide; oxidation of bromide to bromine with potassium permanganate and subsequent reaction with acetone, to give the volatile bromoacetone; quantification of bromoacetone by capillary gas chromatography. The method can also be used with small sample volumes appropriate for paediatric applications. Thermodynamic arguments are used to calculate the equilibria involved. The accuracy and reproducibility of the method have been determined. Finally, the bromide space of six volunteers was determined and the clearance of the bromide by the kidneys was investigated.
已开发出一种灵敏的溴化物测定方法，该方法包括以下步骤：溴化物的分离（纯化）；用高锰酸钾将溴化物氧化为溴，随后与丙酮反应，生成挥发性溴丙酮；通过毛细管气相色谱法对溴丙酮进行定量。该方法也可用于适合儿科应用的小样本量。利用热力学论据来计算所涉及的平衡。已确定了该方法的准确性和重现性。最后，测定了六名志愿者的溴化物分布容积，并研究了肾脏对溴化物的清除情况。
