Mukkur T K, Walker K H, Jones D, Wronski E, Love D N
CSIRO Division of Animal Health, McMaster Laboratory, Glebe, N.S.W., Australia.
Comp Immunol Microbiol Infect Dis. 1991;14(3):243-56. doi: 10.1016/0147-9571(91)90005-x.
Mice immunized with an aromatic-dependent (aro-) S. dublin strain CS101 by either the intraperitoneal (i.p.) or oral route, were protected against oral challenge with a virulent S. dublin strain CS90, the degree of protection being the greatest when mice had received 3 immunizing doses at weekly intervals. Mice immunized with an aromatic-dependent (aro-) S. typhimurium strain CS332 by the i.p. or oral routes were protected against challenge with virulent S. dublin strain CS90 at 1 or 2 weeks but not at 3 or 4 weeks post-immunization. Mice immunized with 1 dose of aro- S. dublin strain CS101 by the i.p. route developed low levels of lipopolysaccharide (LPS) and flagellin-specific antibody but no delayed-type hypersensitivity (DTH) whereas those immunized with 2 or 3 doses developed significantly higher antibody titres and DTH. In contrast, mice immunized by the oral route developed neither significant antibody response nor DTH. The aro- S. dublin strain CS101 could not be detected beyond day 28 post-inoculation in visceral organs including liver, spleen, mesentery, small intestine, caecum or large intestine of mice inoculated by the i.p. route or in mice inoculated by the oral route with the exception of day 42 post-inoculation. Challenge of mice previously immunized with 3 doses of the aro- S. dublin strain CS101 by the i.p. or oral route with virulent S. dublin strain CS90 resulted in their rapid clearance from the above visceral organs. Calves immunized with the aro- S. dublin strain CS101 by either the intramuscular (i.m.) or oral routes were significantly protected against oral challenge with virulent S. dublin strain CS90. In contrast to the observations in mice, somatic (O) and flagellar (H) antibody titres of calves immunized by either route were negligible as were anti-LPS antibody titres. However, flagellin-specific antibody titres were higher in calves immunized by the i.m. than the oral route. These results indicate that the protection observed in immunized mice or calves against oral challenge with virulent S. dublin was unlikely to have been mediated by humoral salmonella-specific immune mechanism(s).
通过腹腔内（i.p.）或口服途径用芳香族依赖性（aro-）都柏林沙门氏菌菌株CS101免疫的小鼠，对强毒都柏林沙门氏菌菌株CS90的口服攻击具有抵抗力，当小鼠每隔一周接受3次免疫剂量时，保护程度最大。通过腹腔内或口服途径用芳香族依赖性（aro-）鼠伤寒沙门氏菌菌株CS332免疫的小鼠，在免疫后1或2周对强毒都柏林沙门氏菌菌株CS90的攻击具有抵抗力，但在免疫后3或4周则没有。通过腹腔内途径用1剂量的aro-都柏林沙门氏菌菌株CS101免疫的小鼠产生低水平的脂多糖（LPS）和鞭毛蛋白特异性抗体，但没有迟发型超敏反应（DTH），而用2或3剂量免疫的小鼠产生明显更高的抗体滴度和DTH。相比之下，通过口服途径免疫的小鼠既没有显著的抗体反应也没有DTH。在接种后28天内，在内脏器官（包括肝脏、脾脏、肠系膜、小肠、盲肠或大肠）中，通过腹腔内途径接种的小鼠或通过口服途径接种的小鼠（接种后42天除外）中，均未检测到aro-都柏林沙门氏菌菌株CS101。用强毒都柏林沙门氏菌菌株CS90对先前通过腹腔内或口服途径用3剂量的aro-都柏林沙门氏菌菌株CS101免疫的小鼠进行攻击，导致它们从上述内脏器官中迅速清除。通过肌肉内（i.m.）或口服途径用aro-都柏林沙门氏菌菌株CS101免疫的小牛，对强毒都柏林沙门氏菌菌株CS90的口服攻击具有显著的抵抗力。与在小鼠中的观察结果相反，通过任何一种途径免疫的小牛的体细胞（O）和鞭毛（H）抗体滴度以及抗LPS抗体滴度都可以忽略不计。然而，通过肌肉内途径免疫的小牛的鞭毛蛋白特异性抗体滴度高于口服途径。这些结果表明，在免疫小鼠或小牛中观察到的对强毒都柏林沙门氏菌口服攻击的保护作用不太可能是由体液性沙门氏菌特异性免疫机制介导的。
