Mukkur T K, Walker K H, Stocker B A
CSIRO Division of Animal Health, McMaster Laboratory, Glebe, NSW, Australia.
Vet Microbiol. 1991 Oct;29(2):181-94. doi: 10.1016/0378-1135(91)90125-y.
The generation of aromatic-dependent (aro-) Salmonella havana (Group G2, 01, 13, 23) from a smooth wild-type parent strain by transduction with phage P1 is reported. Mice immunized with this live aro- S. havana strain (CS234) by the intraperitoneal (i.p.) route were protected against challenge with wild-type S. havana, whereas those immunized by the oral route were not. Mice immunized with two doses of formalin-killed aro- S. havana by the i.p. route were also unprotected, in spite of high antibody titers. However, only those mice immunized with live aro- S. havana by the i.p. route developed significant delayed-type hypersensitivity. Following i.p. inoculation in mice, the aro- S. havana strain CS234 was detected in the liver, spleen and mesenteric lymph nodes on day 9 but not on day 15 post-inoculation (p.i.). On the other hand, when mice were inoculated with the parent wild-type strain (CS4) or the aro- derivative strain CS234 by the oral route, the organisms were recovered from the mesenteric lymph nodes and intestine only on day 3 but not on day 6 post-inoculation. In sheep inoculated with the aro- strain CS234 in the gastroc muscle, organisms were recovered from the muscle, and popliteal and medial iliac lymph nodes for up to 21 but not 28 days p.i. However, no mutant organisms were recovered from liver, spleen, mesenteric lymph nodes or faeces. In orally-inoculated sheep, the mutant organisms were recovered from the mesenteric lymph nodes, rumen, intestinal contents, and faeces up to 14-21 days post-inoculation but not at 28 days. When sheep immunised with the aro- S. havana strain CS234 by the intramuscular or oral route were challenged with the parent wild-type S. havana strain CS4 by the oral route, the latter strain was detectable in the mesenteric lymph nodes and faeces of immune sheep up to 14 days post-challenge in contrast with the non-immune sheep, where the challenge strain was detectable even at 28 days post-challenge. Only sheep immunized by the intramuscular route developed high antibody levels and delayed-type hypersensitivity.
据报道，通过用噬菌体P1转导，从光滑的野生型亲本菌株产生了芳香族依赖性（aro-）哈瓦那沙门氏菌（G2组，01、13、23）。通过腹腔内（i.p.）途径用这种活的aro-哈瓦那沙门氏菌菌株（CS234）免疫的小鼠对野生型哈瓦那沙门氏菌的攻击具有抵抗力，而通过口服途径免疫的小鼠则没有。通过i.p.途径用两剂福尔马林灭活的aro-哈瓦那沙门氏菌免疫的小鼠也没有受到保护，尽管抗体滴度很高。然而，只有通过i.p.途径用活的aro-哈瓦那沙门氏菌免疫的小鼠出现了明显的迟发型超敏反应。在小鼠腹腔内接种后，在接种后第9天在肝脏、脾脏和肠系膜淋巴结中检测到aro-哈瓦那沙门氏菌菌株CS234，但在接种后第15天未检测到。另一方面，当通过口服途径给小鼠接种亲本野生型菌株（CS4）或aro-衍生菌株CS234时，仅在接种后第3天从肠系膜淋巴结和肠道中回收了这些细菌，而在接种后第6天未回收。在通过胃肌接种aro-菌株CS234的绵羊中，在接种后长达21天但不是28天从肌肉、腘窝和髂内侧淋巴结中回收了细菌。然而，在肝脏、脾脏、肠系膜淋巴结或粪便中未回收突变细菌。在口服接种的绵羊中，在接种后长达14 - 21天从肠系膜淋巴结、瘤胃、肠内容物和粪便中回收了突变细菌，但在28天时未回收。当通过肌肉内或口服途径用aro-哈瓦那沙门氏菌菌株CS234免疫的绵羊通过口服途径用亲本野生型哈瓦那沙门氏菌菌株CS4攻击时，与未免疫的绵羊相比，在攻击后长达14天在免疫绵羊 的肠系膜淋巴结和粪便中可检测到后一种菌株，在未免疫绵羊中，即使在攻击后28天也可检测到攻击菌株。只有通过肌肉内途径免疫的绵羊产生了高抗体水平和迟发型超敏反应。
