Cho J W, Sauer H W
Department of Biology, Texas A&M University, College Station 77843-3258.
Eur J Cell Biol. 1994 Oct;65(1):94-102.
A universal model of the control of the cell cycle in eukaryotic organisms has emerged from the discovery that MPF (maturation or mitosis promoting factor) is a heterodimer consisting of a catalytic subunit (p34cdc2) and a regulatory subunit (mitotic cyclin) encoded by a pair of conserved genes. A prominent feature of the periodic activation of the protein kinase p34cdc2 is the gradual accumulation of cyclin in interphase and its abrupt degradation in mitosis, which is believed to be required for inactivation of MPF and exit from mitosis. Utilizing the precise natural synchrony of mitosis of the plasmodium of the myxomycete Physarum, the high affinity of the p34cdc2/cyclin B complex to p13suc1 Sepharose beads, and immunological reagents including three different anticyclin B antibodies and the anti-PSTAIR antibody, a transient histone H1 kinase activation but not fluctuation in the abundance of cyclin B have been detected during mitosis. It is argued that cyclin degradation may be required for cytokinesis and/or postmitotic controls of cell proliferation in G1 phase and cell-to-cell signaling in development but not for the inactivation of histone H1 kinase in mitosis.
真核生物细胞周期调控的通用模型源于一项发现，即MPF（成熟促进因子或有丝分裂促进因子）是一种异源二聚体，由一对保守基因编码的催化亚基（p34cdc2）和调节亚基（有丝分裂周期蛋白）组成。蛋白激酶p34cdc2周期性激活的一个显著特征是周期蛋白在间期逐渐积累，在有丝分裂时突然降解，这被认为是MPF失活和退出有丝分裂所必需的。利用黏菌绒泡菌原质团有丝分裂精确的自然同步性、p34cdc2/周期蛋白B复合物对p13suc1琼脂糖珠的高亲和力以及包括三种不同的抗周期蛋白B抗体和抗PSTAIR抗体在内的免疫试剂，在有丝分裂期间检测到了组蛋白H1激酶的短暂激活，但未检测到周期蛋白B丰度的波动。有人认为，周期蛋白降解可能是胞质分裂和/或G1期细胞增殖的有丝分裂后调控以及发育过程中细胞间信号传导所必需的，但不是有丝分裂中组蛋白H1激酶失活所必需的。
