一种芳香化酶假基因在牛胎盘中被转录。

An aromatase pseudogene is transcribed in the bovine placenta.

作者信息

Fürbass R, Vanselow J

机构信息

Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, Germany.

出版信息

Gene. 1995 Mar 10;154(2):287-91. doi: 10.1016/0378-1119(94)00754-g.

DOI:10.1016/0378-1119(94)00754-g

PMID:7890179

Abstract

The present investigation is focused on a new, truncated bovine aromatase cytochrome P-450-encoding transcript (bCyp19) which was repeatedly isolated from a placental lambda gt10 cDNA library. Different cDNA probes derived from bCyp19 were used during screenings. The deviant transcript contains areas of considerable sequence homology (89-98%) corresponding to exons II, III, V, VIII and IX of bCyp19. Exons VI and VII are missing. Exon IV is replaced by a bovine repeat sequence motif. Numerous translation stop codons occur within all reading frames, thus suggesting that the transcript does not encode a functional protein. The gene was detected by PCR in the genomes of all animals investigated (n = 38). The experiments demonstrate that the bovine genome contains a non-functional copy (pseudogene, Cyp19 phi) of bCyp19 that is transcribed together with the bCyp19 messenger in the bovine post-parturition placenta.

摘要

本研究聚焦于一种新的、截短的牛芳香化酶细胞色素P-450编码转录本（bCyp19），该转录本是从胎盘λgt10 cDNA文库中多次分离得到的。在筛选过程中使用了源自bCyp19的不同cDNA探针。该异常转录本包含与bCyp19的外显子II、III、V、VIII和IX具有相当高序列同源性（89 - 98%）的区域。外显子VI和VII缺失。外显子IV被一个牛重复序列基序所取代。在所有阅读框内都出现了大量翻译终止密码子，因此表明该转录本不编码功能性蛋白质。通过PCR在所有被研究动物（n = 38）的基因组中检测到了该基因。实验表明，牛基因组包含bCyp19的一个无功能拷贝（假基因，Cyp19 phi），它与牛产后胎盘内的bCyp19信使RNA一起被转录。