Pommier de Santi P, Vervloet D, Vellieux P, Payan H, Charpin J
Int Arch Allergy Appl Immunol. 1978;57(5):462-71. doi: 10.1159/000232138.
Leukocytes from subjects allergic to Dermatophagoides pteronyssinus were incubated for 20 min with a solution of D. pteronyssinus extracts. Histamine release was measured at 0, 3, 10 and 20 min. Simultaneously, samples were treated for electron microscopy in such a way as to correlate histamine release and the morphological aspects of basophil leukocytes. The principal features accompanying histamine release were: a progressive activation of the cytoplasmic membrane which showed long processes, densification of the mitochondria, fusion of granulations, progressive dissolution and exocytosis of the contents of the granulations, short segments of rough endoplasmic reticulum, active Golgi apparatus, and thin membrane-bound granules suggesting resynthesis of mediators.
将对屋尘螨过敏的受试者的白细胞与屋尘螨提取物溶液孵育20分钟。在0、3、10和20分钟时测量组胺释放量。同时，对样本进行电子显微镜处理，以便将组胺释放与嗜碱性粒细胞的形态学特征相关联。伴随组胺释放的主要特征有：细胞质膜逐渐激活，呈现出长突起；线粒体致密化；颗粒融合；颗粒内容物逐渐溶解并发生胞吐作用；粗面内质网短片段；高尔基体活跃；以及薄的膜结合颗粒，提示介质的重新合成。
