Jones B B, Reznikoff W S
J Bacteriol. 1976 Oct;128(1):283-9. doi: 10.1128/jb.128.1.283-289.1976.
A lysogen of a wild-type strain of Escherichia coli K-12 carrying a heat-inducible lambda-phi80 hybrid prophage was induced to yield transducing phages carrying all of the structural genes of the tryptophan operon. The presence or absence of elements of the trp regulatory region was determined by examining the effects of lambda genes N and cI on trp gene expression. The phages were further characterized by transduction studies and by examining anthranilate synthetase (EC 4.1.3.27) (TRYPE+D) synthesis in the presence of the lambda cI product. A number of phages deleted for the trp promoter were found. Recombination studies between trpOc bacteria and the transducing phages have yielded information that can be used to order the trp end points of some phages and to provide an estimate of the size of the trp promoter region.
携带热诱导型λ-φ80杂交原噬菌体的大肠杆菌K-12野生型菌株的溶原菌被诱导产生携带色氨酸操纵子所有结构基因的转导噬菌体。通过检测λ基因N和cI对trp基因表达的影响来确定trp调控区元件的存在与否。通过转导研究以及在λ cI产物存在的情况下检测邻氨基苯甲酸合成酶(EC 4.1.3.27)(TRYPE+D)的合成,对这些噬菌体进行了进一步表征。发现了一些缺失trp启动子的噬菌体。trpOc细菌与转导噬菌体之间的重组研究提供了可用于确定某些噬菌体trp端点顺序并估计trp启动子区域大小的信息。