Kikuchi K, Kadono T, Takehara K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Dermatology. 1995;190(1):4-8. doi: 10.1159/000246625.
We investigated the effects of several growth factors on [3H]thymidine incorporation and procollagen type I carboxyterminal propeptide (P1CP) production, which reflects type I collagen metabolism, in keloid and normal fibroblasts.
Six fibroblast cell strains, derived from keloid or normal skin, exhibited similar growth responses to platelet-derived growth factor, transforming growth factor beta 1 (TGF-beta 1), gamma-interferon (gamma-IFN) and histamine. In contrast, keloid fibroblasts showed significantly greater growth response to epidermal growth factor (EGF) than normal fibroblasts. P1CP production was 4.4 times higher in 6 strains of keloid fibroblasts than in 6 controls. Treatment with gamma-IFN (100 U/ml) decreased P1CP production in both groups; the effect was significantly greater in keloid fibroblasts. TGF-beta 1 treatment upregulated P1CP production in both groups. Treatment with histamine increased P1CP production in keloid fibroblasts, although it did not change that in the controls.
EGF and histamine may play some role in the development of keloids.
我们研究了几种生长因子对瘢痕疙瘩和成纤维细胞中[3H]胸苷掺入以及反映I型胶原代谢的I型前胶原羧基末端前肽（P1CP）产生的影响。
从瘢痕疙瘩或正常皮肤中获取的6种成纤维细胞株，对血小板衍生生长因子、转化生长因子β1（TGF-β1）、γ干扰素（γ-IFN）和组胺表现出相似的生长反应。相比之下，瘢痕疙瘩成纤维细胞对表皮生长因子（EGF）的生长反应明显大于正常成纤维细胞。6株瘢痕疙瘩成纤维细胞的P1CP产生量比6个对照高4.4倍。用γ-IFN（100 U/ml）处理可降低两组的P1CP产生量；在瘢痕疙瘩成纤维细胞中的作用明显更大。TGF-β1处理上调了两组的P1CP产生量。组胺处理增加了瘢痕疙瘩成纤维细胞中的P1CP产生量，尽管它对对照组没有影响。
EGF和组胺可能在瘢痕疙瘩的形成中起一定作用。
