Maria D A, Pagliarini R C, Mistrone R J, Pozzi D H, Zyngier S
Disciplina de Hematologia e Hemoterapia, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1994 Jun;27(6):1343-6.
In the present study we characterized the cytotoxicity of Wistar rat mononuclear cells from 21 animals which received 10(6) Walker 256 tumor cells by the subcutaneous route. All animals developed the tumor. Cytotoxicity was studied 15 days after inoculation using spleen, thymus and lymph node T lymphocytes as well as macrophages from the peritoneal cavity. A Walker 256 tumor cell suspension and tumor cells in culture (YAC-1) were labelled with 51Cr and used as target cells, according to the Herberman technique and a gamma counter was used for counting. Anti-Walker cell cytotoxicity was significantly decreased in T lymphocytes from the spleen (9.6% vs 51.1% for the control) and thymus (11.5% vs 38.2% for the control), whereas no difference was observed for lymph nodes (41.2% vs 49.5% for the control) or macrophages (43.4% vs 46.3% for the control). Anti-YAC-1 cytotoxicity was significantly decreased in T lymphocytes from all lymphoid organs compared to control: 23.6% vs 42.8% for the spleen, 22.6% vs 41.1% for the thymus, 26.6% vs 42.1% for lymph nodes, and 27.1% vs 46.3% for macrophages. No correlation was observed between tumor weight, and anti-Walker cytotoxicity or anti-YAC-1 cytotoxicity.
在本研究中，我们对21只经皮下接种10⁶个Walker 256肿瘤细胞的Wistar大鼠的单核细胞的细胞毒性进行了表征。所有动物均长出了肿瘤。在接种后15天，使用脾脏、胸腺和淋巴结中的T淋巴细胞以及腹腔巨噬细胞研究细胞毒性。根据赫伯曼技术，将Walker 256肿瘤细胞悬液和培养中的肿瘤细胞（YAC-1）用⁵¹Cr标记并用作靶细胞，并用γ计数器进行计数。脾脏T淋巴细胞（对照组为51.1%，实验组为9.6%）和胸腺T淋巴细胞（对照组为38.2%，实验组为11.5%）中抗Walker细胞的细胞毒性显著降低，而淋巴结（对照组为49.5%，实验组为41.2%）和巨噬细胞（对照组为46.3%，实验组为43.4%）中未观察到差异。与对照组相比，所有淋巴器官中的T淋巴细胞对YAC-1的细胞毒性均显著降低：脾脏为23.6%对42.8%，胸腺为22.6%对41.1%，淋巴结为26.6%对42.1%，巨噬细胞为27.1%对46.3%。未观察到肿瘤重量与抗Walker细胞毒性或抗YAC-1细胞毒性之间的相关性。
