Jenkins M A, O'Leary T D, Guerin M D
Department of Chemical Pathology, Heidelberg Repatriation Hospital, Vic., Australia.
J Chromatogr B Biomed Appl. 1994 Dec 2;662(1):108-12. doi: 10.1016/0378-4347(94)00387-4.
Capillary electrophoresis, as a fully automatable, powerful analytical technique, has the potential for use in clinical laboratories for separation and identification of protein types in normal and abnormal human urine specimens. Abnormal urine specimens containing previously identified proteins, anion-exchange resin treatment and addition of known components were used to identify and determine migration times of the peaks found in the electropherogram of human urine. The correlation coefficients between capillary electrophoresis and the currently used method of high-resolution agarose gel electrophoresis for Bence Jones protein and albumin were found to be 0.95 and 0.93, respectively.
毛细管电泳作为一种完全自动化的强大分析技术，有潜力应用于临床实验室，用于分离和鉴定正常及异常人类尿液标本中的蛋白质类型。含有先前鉴定出的蛋白质的异常尿液标本、阴离子交换树脂处理以及已知成分的添加，被用于识别和确定人类尿液电泳图谱中发现的峰的迁移时间。发现毛细管电泳与目前用于检测本-周蛋白和白蛋白的高分辨率琼脂糖凝胶电泳方法之间的相关系数分别为0.95和0.93。
