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蛋白尿筛查:尿白蛋白测定的理由

Screening for albuminuria: a case for estimation of albumin in urine.

作者信息

BenGershôm E

出版信息

Clin Chem. 1975 Nov;21(12):1795-8.

PMID:1182999
Abstract

The usefulness of bromcresol green for estimating albumin in urine was evaluated by comparison with the Laurell "rocket" technique. In contrast to the bromcresol green method applied for urinary albumin, rather doubtful results were obtained with conventional (Microzone) electrophoresis for albumin and with precipitation techniques for total protein estimation. Albumin estimation with bromcresol green is recommended as a more reliable substitute for total-protein estimations in urine. Limitations of bromcresol green are also pointed out.

摘要

通过与劳雷尔“火箭”技术进行比较,评估了溴甲酚绿用于估算尿白蛋白的效用。与应用于尿白蛋白的溴甲酚绿方法相比,传统的(微量区带)白蛋白电泳和总蛋白估算的沉淀技术得到的结果相当可疑。建议使用溴甲酚绿估算尿白蛋白,作为尿总蛋白估算更可靠的替代方法。同时也指出了溴甲酚绿的局限性。

相似文献

1
Screening for albuminuria: a case for estimation of albumin in urine.蛋白尿筛查:尿白蛋白测定的理由
Clin Chem. 1975 Nov;21(12):1795-8.
2
Urinary albumin determination by the immediate bromcresol green method.
Clin Chim Acta. 1978 Dec 15;90(3):241-8. doi: 10.1016/0009-8981(78)90263-2.
3
An evaluation of the overestimation of serum albumin by bromcresol green.溴甲酚绿对血清白蛋白高估情况的评估。
Am J Clin Pathol. 1978 Mar;69(3):347-50. doi: 10.1093/ajcp/69.1.347.
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Identification and quantitation of human urine proteins by capillary electrophoresis.通过毛细管电泳对人尿蛋白进行鉴定和定量分析。
J Chromatogr B Biomed Appl. 1994 Dec 2;662(1):108-12. doi: 10.1016/0378-4347(94)00387-4.
5
Improved specificity of serum albumin determination and estimation of "acute phase reactants" by use of the bromcresol green reaction.
Clin Chem. 1976 May;22(5):616-22.
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Serum and urine albumin: a progress report on their measurement and clinical significance.血清和尿白蛋白:关于其测定及临床意义的进展报告
Clin Chim Acta. 1997 Feb 3;258(1):3-20. doi: 10.1016/s0009-8981(96)06446-7.
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Routine differential diagnosis of proteinurias by capillary electrophoresis.
Clin Chem Lab Med. 2001 Sep;39(9):784-8. doi: 10.1515/CCLM.2001.129.
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A simple and inexpensive screening test for low protein levels in urine.
Clin Chim Acta. 1997 Feb 17;258(2):231-9. doi: 10.1016/s0009-8981(96)06465-0.
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Continuous-flow analysis for serum albumin, by use of the immediate reaction with bromcresol green.采用溴甲酚绿直接反应法对血清白蛋白进行连续流动分析。
Clin Chem. 1978 Nov;24(11):2036-9.
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Comparison of several methods for semiquantitative determination of urinary protein.几种尿蛋白半定量测定方法的比较
Clin Chem. 1977 May;23(5):876-9.

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