Harbuz M S, Jessop D S, Lightman S L, Chowdrey H S
University Department of Medicine, Bristol Royal Infirmary, UK.
Brain Res. 1994 Dec 19;667(1):6-12. doi: 10.1016/0006-8993(94)91707-8.
It is generally assumed that the stress response of different strains of rat will be identical following exposure to acute stress. In the present study we have examined the activation of the hypothalamo-pituitary-adrenal axis in the Wistar, Sprague-Dawley and CFY strains of rat following exposure to either the predominantly psychological stress of restraint or the physical stress of i.p. hypertonic saline injection. We have investigated the hypothalamic activation of corticotrophin-releasing factor (CRF) and proenkephalin A (PEA) mRNAs in the parvocellular cells of the paraventricular nucleus (PVN) and arginine vasopressin (AVP) in both the magnocellular and parvocellular regions in the PVN following acute stress. In addition we have measured corticosterone as an index of end-point activation. Circulating corticosterone and CRF mRNA were increased in all three strains following either stress. AVP and PEA mRNAs were increased following hypertonic saline but only in the CFY strain following restraint. Overall the relative increase in the parameters measured was greater in the CFY strain of rat than the other strains. These data demonstrate marked differences in response to acute stress in the three strains of rat examined. These varying responses must be taken into consideration when designing or interpreting any study investigating the stress response.
一般认为，不同品系的大鼠在暴露于急性应激后，其应激反应是相同的。在本研究中，我们检测了Wistar、Sprague-Dawley和CFY品系的大鼠在暴露于主要为心理应激的束缚或腹腔注射高渗盐水的物理应激后，下丘脑-垂体-肾上腺轴的激活情况。我们研究了急性应激后室旁核（PVN）小细胞中促肾上腺皮质激素释放因子（CRF）和脑啡肽原A（PEA）mRNA的下丘脑激活情况，以及PVN大细胞和小细胞区域中精氨酸加压素（AVP）的激活情况。此外，我们测量了皮质酮作为终点激活的指标。在两种应激后，所有三个品系的循环皮质酮和CRF mRNA均增加。高渗盐水注射后AVP和PEA mRNA增加，但束缚后仅CFY品系增加。总体而言，所测参数的相对增加在CFY品系大鼠中比其他品系更大。这些数据表明，在所研究的三个品系大鼠中，对急性应激的反应存在显著差异。在设计或解释任何研究应激反应的研究时，必须考虑到这些不同的反应。
