Bastianelli E, Pochet R
Laboratoire d'Histologie, Faculté de Médecine, Université Libre de Bruxelles, Brussels, Belgium.
Neurosci Res. 1993 Jun;17(1):53-61. doi: 10.1016/0168-0102(93)90029-p.
Calcium ions play a critical role in neural development. Insights into the ontogeny of Ca2+ homeostasis were gained by investigating the developmental expression of two E-F hand calcium-binding proteins. Calretinin and calbindin were monitored through their immunoreactivity in the developing chick cerebellum (from E6 to E20). Calbindin was detected from E13 and in Purkinje cells only. Intensity of labelling increased with Purkinje cell development. Calretinin presented a transitory immunoreactivity between E11 and E20 in the internal granular cell layer. This cell layer contains cells which will differentiate into Golgi and granular cells which are calretinin-negative in adult chick cerebellum. Calretinin immunoreactivity presented a peak (both in number of cells and in intensity) at E15 and fell dramatically after E20 while calbindin immunoreactivity was restricted to the Purkinje cells and increased with the development of these cells.
钙离子在神经发育中起着关键作用。通过研究两种E-F手型钙结合蛋白的发育表达，我们对钙离子稳态的个体发生有了深入了解。通过免疫反应性监测了发育中的鸡小脑（从胚胎第6天到第20天）中钙视网膜蛋白和钙结合蛋白的情况。钙结合蛋白在胚胎第13天开始被检测到，并且仅在浦肯野细胞中存在。标记强度随着浦肯野细胞的发育而增加。钙视网膜蛋白在胚胎第11天到第20天期间在内颗粒细胞层呈现短暂的免疫反应性。这个细胞层包含将分化为成年鸡小脑中钙视网膜蛋白阴性的高尔基细胞和颗粒细胞的细胞。钙视网膜蛋白免疫反应性在胚胎第15天达到峰值（细胞数量和强度方面），在胚胎第20天后急剧下降，而钙结合蛋白免疫反应性则局限于浦肯野细胞，并随着这些细胞的发育而增加。
