Redei E, Hilderbrand H, Aird F
Department of Pharmacology, University of Pennsylvania, Philadelphia 19104.
Endocrinology. 1995 Apr;136(4):1813-6. doi: 10.1210/endo.136.4.7895696.
Corticotropin synthesis and secretion is under negative feedback regulation by glucocorticoids. In addition a hypothalamic factor inhibiting ACTH synthesis and secretion, named corticotropin release inhibiting factor (CRIF), has been postulated but not identified. Here we report that transient transfection of a rat prepro-TRH cDNA into the mouse anterior pituitary tumor cell line AtT-20 inhibits the synthesis and secretion of both basal and CRH-stimulated ACTH. Thus, CRIF appears to be encoded by the same precursor as TRH, suggesting a coordinated regulation of pituitary-adrenal and pituitary-thyroid functions.
促肾上腺皮质激素的合成和分泌受糖皮质激素的负反馈调节。此外，已推测存在一种抑制促肾上腺皮质激素合成和分泌的下丘脑因子，称为促肾上腺皮质激素释放抑制因子（CRIF），但尚未确定。在此我们报告，将大鼠前促甲状腺激素释放激素（prepro-TRH）cDNA瞬时转染到小鼠垂体前叶肿瘤细胞系AtT-20中，可抑制基础状态下以及促肾上腺皮质激素释放激素（CRH）刺激下的促肾上腺皮质激素的合成和分泌。因此，CRIF似乎与促甲状腺激素释放激素（TRH）由同一前体编码，提示垂体 - 肾上腺和垂体 - 甲状腺功能存在协同调节。
