通过血凝抑制试验确定的人腺病毒12型、18型和31型之间的关系

Relationship of human adenoviruses 12, 18, and 31 as determined by hemagglutination inhibition.

作者信息

Wigand R, Keller D

出版信息

J Med Virol. 1978;2(2):137-42. doi: 10.1002/jmv.1890020208.

DOI:10.1002/jmv.1890020208

Abstract

Adenoviruses 12 and 31, but not Ad18, agglutinate rat blood cells at high titer, providing suitable blood cells be available and a prolonged contact period of virus with the erythrocytes is allowed. Purified virus particles show direct, and virus-free supernatants show direct and indirect, hemagglutination, ie, enhancement of HA by heterologous antiserum. Hemagglutination inhibition with rabbit antisera shows cross-reactions between Ad12 and Ad31 with titers 4--32 times lower than with homologous antigens; Ad18 antisera react with antigens from both of the other serotypes. No cross-reactions were seen with antisera from other adenoviruses. This suggests an antigenic relationship of the three viruses of subgroup IV in their fiber antigen gamma, in addition to the known relation in the hexon (epsilon), which is apparent in cross-neutralization.

摘要

腺病毒12型和31型（而非腺病毒18型）能以高滴度凝集大鼠血细胞，前提是有合适的血细胞且病毒与红细胞的接触时间足够长。纯化的病毒颗粒显示直接血凝，无病毒的上清液显示直接和间接血凝，即异源抗血清增强血凝作用。用兔抗血清进行的血凝抑制试验表明，腺病毒12型和31型之间存在交叉反应，其效价比同源抗原低4至32倍；腺病毒18型抗血清与其他两种血清型的抗原均有反应。与其他腺病毒的抗血清未观察到交叉反应。这表明除了已知的六邻体（ε）中的关系外，IV亚组的这三种病毒在其纤维抗原γ中也存在抗原关系，这种关系在交叉中和试验中很明显。