Hierholzer J C, Stone Y O, Broderson J R
Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia.
Arch Virol. 1991;121(1-4):179-97. doi: 10.1007/BF01316753.
Reference equine antisera to all 47 serotypes of human adenoviruses presently described have been prepared and evaluated by reciprocal neutralization and hemagglutination-inhibition tests. All tests were carried to endpoint dilutions a minimum of five times in each direction to give accurate values for homologous and heterologous antibody titers. Significant cross-reactions in the horse antisera were compared to similar data obtained from rabbit antisera. Using this analysis, major antigenic relationships exist among types 12-18-31 of subgenus A, types 7-11-14 and 34-35 of subgenus B, types 8-9-10, 10-19-37, 13-38-39, 15-22-42, 20-47, 24-32-33-46, and 29-45 of subgenus D, types 16-4 between subgenera B and E, and types 40-41 of subgenus F. Across all subgenera, types 8, 10, 13, 15, 17, 19, 26, 29, 39, 40, and 43 have antigenic moieties found most frequently in other types, averaging 12 heterologous reactions per type when summing both tests in both directions. Types 20, 30, 32, and 45 exhibit shared determinants slightly less often, with a mean of 8 heterologous reactions per type.
现已制备了针对目前所描述的人类腺病毒全部47个血清型的马参考抗血清，并通过双向中和试验和血凝抑制试验进行了评估。所有试验均进行至终点稀释度，每个方向至少重复5次，以得出同源和异源抗体效价的准确数值。将马抗血清中的显著交叉反应与从兔抗血清获得的类似数据进行了比较。通过这种分析，A亚属的12、18、31型之间，B亚属的7、11、14型和34、35型之间，D亚属的8、9、10型，10、19、37型，13、38、39型，15、22、42型，20、47型，24、32、33、46型，以及29、45型之间，B亚属和E亚属的16、4型之间，F亚属的40、41型之间存在主要抗原关系。在所有亚属中，8、10、13、15、17、19、26、29、39、40和43型具有在其他类型中最常发现的抗原部分，两个方向的两项试验相加时，每种类型平均有12次异源反应。20、30、32和45型出现共同决定簇的频率略低，每种类型平均有8次异源反应。
