Gan K, Sankaran K, Williams M G, Aldea M, Rudd K E, Kushner S R, Wu H C
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
J Bacteriol. 1995 Apr;177(7):1879-82. doi: 10.1128/jb.177.7.1879-1882.1995.
Using a combination of biochemical, physical, and genetic techniques, we have shown that the umpA gene of Escherichia coli is allelic with the lgt (phosphatidylglycerol:prolipoprotein diacylglyceryl transferase) of Salmonella typhimurium. These genes are essential for the viability of the respective organism and exhibit 92.8% sequence identity at the amino acid level. In E. coli, lgt and thyA (thymidylate synthase) form an operon. Thymidylate synthase levels are regulated by transcription from the lgt promoter and by translational coupling.
通过结合生化、物理和遗传技术，我们已证明大肠杆菌的umpA基因与鼠伤寒沙门氏菌的lgt（磷脂酰甘油：前脂蛋白二酰甘油转移酶）等位。这些基因对于各自生物体的生存能力至关重要，并且在氨基酸水平上具有92.8%的序列同一性。在大肠杆菌中，lgt和thyA（胸苷酸合成酶）形成一个操纵子。胸苷酸合成酶的水平受lgt启动子转录和翻译偶联的调控。
