Inoue K, Takeuchi Y, Miki D, Odo S
Kamaishi Laboratories, Marine Biotechnology Institute, Iwate, Japan.
J Biol Chem. 1995 Mar 24;270(12):6698-701. doi: 10.1074/jbc.270.12.6698.
A mussel (Mytilus galloprovincialis) cDNA encoding Mgfp2, a major component of the adhesive plaque that anchors mussels tightly to underwater surfaces was isolated. It encoded a protein mainly consisted of epidermal growth factor-like repeats, containing tyrosine residues that will be converted to 3,4-dihydroxyphenylalanine near C and N termini. Amino acid residues important for cell-cell interaction in other epidermal growth factor-like proteins were, however, not conserved in the structure of Mgfp2. RNA blot analysis on adult tissues showed foot-specific expression of this gene, while the analysis on developing larvae showed that the expression starts with formation of the foot. These results suggest that the function of Mgfp2 has been specialized to form the adhesive plaque.
分离出一种贻贝（Mytilus galloprovincialis）的cDNA，其编码Mgfp2，Mgfp2是将贻贝紧密锚定在水下表面的粘附斑的主要成分。它编码一种主要由表皮生长因子样重复序列组成的蛋白质，在C和N末端附近含有将转化为3,4-二羟基苯丙氨酸的酪氨酸残基。然而，其他表皮生长因子样蛋白中对细胞间相互作用重要的氨基酸残基在Mgfp2的结构中并不保守。对成年组织的RNA印迹分析表明该基因在足部特异性表达，而对发育中的幼虫的分析表明，该表达始于足部的形成。这些结果表明Mgfp2的功能已专门用于形成粘附斑。
