Pascual C, Crespo J F, Quiralte J, Lopez C, Wheeler G, Martin-Esteban M
Servicio de Alergia, Hospital La Paz, Madrid, Spain.
J Allergy Clin Immunol. 1995 Mar;95(3):668-71. doi: 10.1016/s0091-6749(95)70170-2.
In vitro tests for detecting drug-specific IgE would be useful in identifying patients at risk for immediate hypersensitivity reactions to therapeutic doses of a drug.
We attempted to verify that IgE-mediated reactions to erythromycin occur and to identify IgE antibodies specific for erythromycin in serum from a patient who had urticaria immediately after administration of the drug.
Skin prick testing was performed on the patient and five control subjects. Serum from the patient, pooled sera from nonatopic subjects allergic to common aeroallergens, and cord blood controls were analyzed for erythromycin-specific IgE by radioimmunoassay. Sepharose (Pharmacia, Uppsala, Sweden) was used as solid phase covalently linked to erythromycin.
We were able to detect erythromycin-specific IgE antibodies in serum from the patient who had an allergic reaction to this antibiotic, but specific IgE could not be detected in control sera.
Immunologic IgE-mediated reactions to erythromycin do occur, and in vitro diagnosis of such reactions can be made by using Sepharose as a solid phase covalently linked to this drug.
检测药物特异性IgE的体外试验有助于识别对治疗剂量药物发生速发型超敏反应风险的患者。
我们试图证实对红霉素的IgE介导反应的存在，并在一名用药后立即出现荨麻疹的患者血清中鉴定出对红霉素特异性的IgE抗体。
对该患者和五名对照受试者进行皮肤点刺试验。采用放射免疫分析法分析该患者的血清、对常见气传变应原过敏的非特应性受试者的混合血清以及脐血对照中的红霉素特异性IgE。使用共价连接到红霉素的琼脂糖（瑞典乌普萨拉法玛西亚公司）作为固相。
我们能够在对该抗生素发生过敏反应的患者血清中检测到红霉素特异性IgE抗体，但在对照血清中未检测到特异性IgE。
确实存在对红霉素的免疫性IgE介导反应，并且通过使用共价连接到该药物的琼脂糖作为固相可对此类反应进行体外诊断。
