用于生物分子制备和研究的混合扫描透射电子/扫描隧道显微镜系统。

Hybrid scanning transmission electron/scanning tunneling microscope system for the preparation and investigation of biomolecules.

作者信息

Knapp H F, Wyss R, Häring R, Henn C, Guckenberger R, Engel A

机构信息

Maurice E. Müller Institute for Microscopic Structural Biology, Biozentrum, University of Basel, Switzerland.

出版信息

J Microsc. 1995 Jan;177(Pt 1):31-42. doi: 10.1111/j.1365-2818.1995.tb03531.x.

DOI:10.1111/j.1365-2818.1995.tb03531.x

PMID:7897646

Abstract

A hybrid scanning transmission electron/scanning tunneling microscope vacuum system is introduced, which allows freeze drying and metal coating of biological samples and their simultaneous observation by scanning transmission electron microscopy and scanning tunnelling microscopy (STM). Different metal coatings and STM tips were analysed to obtain the highest possible resolution for such a system. Bovine liver catalase was used as a test sample and the STM results are compared to a molecular scale model.

摘要

介绍了一种混合扫描透射电子/扫描隧道显微镜真空系统，该系统允许对生物样品进行冷冻干燥和金属镀膜，并通过扫描透射电子显微镜和扫描隧道显微镜（STM）对其进行同步观察。分析了不同的金属镀膜和STM探针，以获得该系统尽可能高的分辨率。使用牛肝过氧化氢酶作为测试样品，并将STM结果与分子尺度模型进行比较。