Colliex C, Mory C
Laboratoire de Physique des Solides, Université Paris-Sud, Orsay, France.
Biol Cell. 1994;80(2-3):175-80. doi: 10.1111/j.1768-322x.1994.tb00928.x.
The design of the scanning transmission electron microscope (STEM) has been conceived to optimize its detection efficiency of the different elastic and inelastic signals resulting from the interaction of the high energy primary electrons with the specimen. Its potential use to visualize and measure biological objects was recognized from the first studies by Crewe and coworkers in the seventies. Later the real applications have not followed the initial hopes. The purpose of the present paper is to describe how the instrument has practically evolved and recently begun to demonstrate all its potentialities for quantitative electron microscopy of a wide range of biological specimens, from freeze-dried isolated macromolecules to unstained cryosections. Emphasis will be put on the mass-mapping, multi-signal and elemental mapping modes which are unique features of the STEM instruments.
扫描透射电子显微镜（STEM）的设计旨在优化其对高能初级电子与样品相互作用产生的不同弹性和非弹性信号的检测效率。早在七十年代，克鲁和他的同事们的初步研究就认识到了它在可视化和测量生物物体方面的潜在用途。然而，后来实际应用并未达到最初的期望。本文的目的是描述该仪器实际上是如何发展的，以及最近它如何开始展现出对从冷冻干燥的分离大分子到未染色冷冻切片等广泛生物标本进行定量电子显微镜分析的所有潜力。重点将放在STEM仪器独有的质量映射、多信号和元素映射模式上。
