Canfield L M, Chytil F
J Nutr. 1978 Aug;108(8):1343-7. doi: 10.1093/jn/108.8.1343.
Prolonged lysine deficiency resulted in inhibition of growth of the rat and in lowered levels of albumin and beta-globulins, but not of total liver proteins. The apparent incorporation of [3H]lysine into total and chromosomal liver proteins, however, was increased in lysine deficient rats relative to pair-fed controls. The concentration of free lysine in neither serum nor liver was altered in lysine deficient rats. The specific radioactivity of liver free lysine 2 hours after administration of [3H]lysine was noticeably elevated in the deficient rats. However, when the pool of liver free lysine is considered, synthesis of all proteins studied was inhibited. The largest inhibitory effect was seen in serum albumin and total liver protein synthesis. Inhibition of snythesis of nuclear chromatin protein was less pronounced. The data indicate an adaptive mechanism operates to preserve liver lysine in rats fed lysine deficient diets for a prolonged period. In spite of retention of lysine, the synthesis of liver and serum proteins was inhibited.
长期赖氨酸缺乏导致大鼠生长受抑制，白蛋白和β-球蛋白水平降低，但肝脏总蛋白水平未受影响。然而，相对于配对喂养的对照组，赖氨酸缺乏的大鼠肝脏中[3H]赖氨酸掺入总蛋白和染色体蛋白的表观量增加。赖氨酸缺乏的大鼠血清和肝脏中的游离赖氨酸浓度均未改变。给予[3H]赖氨酸2小时后，缺乏组大鼠肝脏游离赖氨酸的比放射性显著升高。然而，当考虑肝脏游离赖氨酸池时，所有研究蛋白质的合成均受到抑制。血清白蛋白和肝脏总蛋白合成受到的抑制作用最大。核染色质蛋白合成的抑制作用不那么明显。数据表明，在长期喂食赖氨酸缺乏饮食的大鼠中，存在一种适应性机制来保存肝脏中的赖氨酸。尽管赖氨酸得以保留，但肝脏和血清蛋白的合成仍受到抑制。
