Canfield L M, Chytil F
J Nutr. 1978 Aug;108(8):1336-42. doi: 10.1093/jn/108.8.1336.
Prolonged dietary lysine deficiency induced by feeding male weanling rats a diet low in lysine for 6 weeks resulted in substantial impairment of growth as compared with control rats and in alterations in the morphology of the nucleus of hepatocytes. Electronmicrographs of liver nuclei from lysine deficient rats showed an increased incidence of binucleated cells, reduction in numbers of nucleoli, changes in the position and size of nucleoli within the nucleus and altered patterns of chromatin aggregation in lysine deficient rats. Mass ratios of nonhistone proteins to DNA in liver chromatin from lysine deficient rats were higher than those of controls and electrophoretic patterns of chromatin nonhistone proteins were altered. In particular, one high molecular weight fraction of these proteins showing the same mobility as myosin was substantially enriched. The histone: DNA ratio and histone electrophoretic patterns, however, appeared to be unchanged. Incorporation of [3H] thymidine into DNA was increased, but the nuclear content of DNA was unchanged in lysine deficient animals.
给雄性断奶大鼠喂食低赖氨酸饮食6周，由此导致的长期饮食赖氨酸缺乏，与对照大鼠相比，导致生长显著受损，并使肝细胞的细胞核形态发生改变。赖氨酸缺乏大鼠肝脏细胞核的电子显微镜照片显示，双核细胞的发生率增加，核仁数量减少，核仁在细胞核内的位置和大小发生变化，且赖氨酸缺乏大鼠的染色质聚集模式改变。赖氨酸缺乏大鼠肝脏染色质中，非组蛋白与DNA的质量比高于对照组，染色质非组蛋白的电泳图谱也发生改变。特别是，这些蛋白质中有一个与肌球蛋白迁移率相同的高分子量组分显著富集。然而，组蛋白与DNA的比例以及组蛋白电泳图谱似乎未发生变化。在赖氨酸缺乏的动物中，[3H]胸苷掺入DNA的量增加，但细胞核中的DNA含量未变。
