一种制备用于培养的成肌细胞的简化程序。
A simplified procedure for preparing myogenic cells for culture.
作者信息
Caplan A I
出版信息
J Embryol Exp Morphol. 1976 Aug;36(1):175-81.
PMID:789809
Abstract
A procedure involving mechanical agitation referred to as vortexing is compared to a trypsin procedure for obtaining myogenic cells for culture. The vortex procedure appears to be at least as useful as the trypsin procedure and has several advantages including speed, the elimination of chemical disruptive agent, elimination of collagen coating of culture dishes and earlier onset of fusion.
摘要
将一种称为涡旋的机械搅拌方法与胰蛋白酶法进行比较，以获取用于培养的成肌细胞。涡旋法似乎至少与胰蛋白酶法一样有用，并且具有几个优点，包括速度快、无需化学破坏剂、无需对培养皿进行胶原包被以及融合开始时间更早。
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