• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种制备用于培养的成肌细胞的简化程序。

A simplified procedure for preparing myogenic cells for culture.

作者信息

Caplan A I

出版信息

J Embryol Exp Morphol. 1976 Aug;36(1):175-81.

PMID:789809
Abstract

A procedure involving mechanical agitation referred to as vortexing is compared to a trypsin procedure for obtaining myogenic cells for culture. The vortex procedure appears to be at least as useful as the trypsin procedure and has several advantages including speed, the elimination of chemical disruptive agent, elimination of collagen coating of culture dishes and earlier onset of fusion.

摘要

将一种称为涡旋的机械搅拌方法与胰蛋白酶法进行比较，以获取用于培养的成肌细胞。涡旋法似乎至少与胰蛋白酶法一样有用，并且具有几个优点，包括速度快、无需化学破坏剂、无需对培养皿进行胶原包被以及融合开始时间更早。

相似文献

1
A simplified procedure for preparing myogenic cells for culture.一种制备用于培养的成肌细胞的简化程序。
J Embryol Exp Morphol. 1976 Aug;36(1):175-81.
2
Changes in ribosome-polyribosome balances in chick muscle cells during tissue dissociation, development in culture, and exposure to simplified culture-medium.鸡肌肉细胞在组织解离、培养发育以及暴露于简化培养基过程中核糖体-多核糖体平衡的变化
J Cell Physiol. 1971 Apr;77(2):145-56. doi: 10.1002/jcp.1040770204.
3
Dissociation of chick embryo spinal ganglia and the effects on cell yields by the mouse 7S nerve growth factor protein.鸡胚脊髓神经节的解离以及小鼠7S神经生长因子蛋白对细胞产量的影响。
Neurobiology. 1972;2(3):106-22.
4
Comparison of skeletal muscle monolayer cultures initiated with cells dissociated by the vortex and trypsin methods.采用涡旋法和胰蛋白酶法解离细胞起始的骨骼肌单层培养物的比较。
In Vitro. 1976 Aug;12(8):564-70. doi: 10.1007/BF02797440.
5
DNA synthesis, mitosis and fusion of myocardial cells.心肌细胞的DNA合成、有丝分裂和融合
J Morphol. 1972 Aug;137(4):417-31. doi: 10.1002/jmor.1051370403.
6
Cell separation by velocity sedimentation of postnatal mouse cerebellum.
J Cell Physiol. 1973 Apr;81(2):271-9. doi: 10.1002/jcp.1040810215.
7
[Comparison of promoting the proliferation and fusion of myoblast between conditioned media from fibroblasts of chick embryo and fetal mouse].[鸡胚成纤维细胞与胎鼠成纤维细胞条件培养基促进成肌细胞增殖与融合的比较]
Sheng Li Xue Bao. 1989 Apr;41(2):191-5.
8
Myogenic cells derived from murine sarcoma induced mouse tumours: DNA synthesis and the effects of simian virus 40 infection.源自鼠肉瘤的成肌细胞诱发小鼠肿瘤：DNA合成及猿猴病毒40感染的影响。
Exp Cell Res. 1973 Apr;79(1):231-5.
9
[Intercellular interactions preceding muscle cell fusion].[肌肉细胞融合之前的细胞间相互作用]
Tsitologiia. 1985 Dec;27(12):1404-7.
10
Myogenic cells derived from murine sarcoma virus induced mouse tumours: DNA synthesis and the effects of Simian virus 40 infection.源自鼠肉瘤病毒诱导的小鼠肿瘤的肌源性细胞：DNA合成及猿猴病毒40感染的影响
Exp Eye Res. 1973 Apr;79(1):231-5.

引用本文的文献

1
A simplified but robust method for the isolation of avian and mammalian muscle satellite cells.一种用于分离禽类和哺乳动物肌肉卫星细胞的简化而可靠的方法。
BMC Cell Biol. 2012 Jun 21;13:16. doi: 10.1186/1471-2121-13-16.
2
Pluripotent mesenchymal stem cells reside within avian connective tissue matrices.多能间充质干细胞存在于鸟类结缔组织基质中。
In Vitro Cell Dev Biol Anim. 1993 Sep;29A(9):723-36. doi: 10.1007/BF02631429.
3
Identity of the core proteins of the large chondroitin sulphate proteoglycans synthesized by skeletal muscle and prechondrogenic mesenchyme.
由骨骼肌和软骨形成前间充质合成的大硫酸软骨素蛋白聚糖核心蛋白的特性。
Biochem J. 1994 Feb 15;298 ( Pt 1)(Pt 1):51-60. doi: 10.1042/bj2980051.
4
Expression of type I and III collagen genes during differentiation of embryonic chicken myoblasts in culture.培养的胚胎鸡成肌细胞分化过程中I型和III型胶原基因的表达
Mol Cell Biol. 1984 Aug;4(8):1483-92. doi: 10.1128/mcb.4.8.1483-1492.1984.
5
Development of the cyclic AMP response to parathyroid hormone and prostaglandin E2 in the embryonic chick limb.胚胎期鸡胚肢体中对甲状旁腺激素和前列腺素E2的环磷酸腺苷反应的发育
Calcif Tissue Int. 1981;33(6):641-8. doi: 10.1007/BF02409502.
6
Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts.佛波醇肉豆蔻酸酯乙酸酯处理的软骨细胞和成纤维细胞中β-肌动蛋白基因表达的改变
Mol Cell Biol. 1985 Jun;5(6):1425-33. doi: 10.1128/mcb.5.6.1425-1433.1985.
7
Changes in the H-1 histone complement during myogenesis. II. Regulation by differential coupling of H-1 variant mRNA accumulation to DNA replication.肌生成过程中H-1组蛋白补体的变化。II. H-1变体mRNA积累与DNA复制的差异偶联调控。
J Cell Biol. 1985 Jul;101(1):175-81. doi: 10.1083/jcb.101.1.175.
8
Changes in the H-1 histone complement during myogenesis. I. Establishment by differential coupling of H-1 species synthesis to DNA replication.肌生成过程中H-1组蛋白补体的变化。I. 通过H-1组蛋白合成与DNA复制的差异偶联建立。
J Cell Biol. 1985 Jul;101(1):167-74. doi: 10.1083/jcb.101.1.167.
9
Fibroblasts promote the formation of a continuous basal lamina during myogenesis in vitro.成纤维细胞在体外肌生成过程中促进连续基膜的形成。
J Cell Biol. 1986 Mar;102(3):740-7. doi: 10.1083/jcb.102.3.740.
10
Analysis of the upstream regions governing expression of the chicken cardiac troponin T gene in embryonic cardiac and skeletal muscle cells.鸡心肌肌钙蛋白T基因在胚胎心脏和骨骼肌细胞中表达的上游调控区域分析。
J Cell Biol. 1988 Aug;107(2):573-85. doi: 10.1083/jcb.107.2.573.