Lin W C, Tan T W
Department of Pharmacology, China Medical College, Taichung city, Taiwan.
J Ethnopharmacol. 1994 Dec;44(3):171-9. doi: 10.1016/0378-8741(94)01184-2.
We examined the mechanism of the protective effect of San-huang-xie-xin-tang (SHXT), a traditional oriental drug, on the gastric mucosa. SHXT, given intraduodenally, inhibited the gastric secretion in pylorus-ligated rats. SHXT, given orally 30 min before the administration of aspirin, HCl-aspirin and ethanol, protected the gastric mucosa from these agent-induced gastric lesions, although it had no effect on the gastric lesions induced by indomethacin and water immersion stress. Oral administration of SHXT increased gastric contents (muscle relaxation) when they were measured 30 min after SHXT administration. SHXT also inhibited carbachol-contracted gastric muscle in vitro. Neither increased gastric contents nor inhibited ethanol lesions of SHXT were observed when these were tested 4 h after SHXT administration. Pretreatment with indomethacin which is a prostaglandin synthesis inhibitor, and N-ethylmaleimide which is a sulfhydryl blocker did not influence either the increased gastric contents or inhibited ethanol lesions of SHXT. These results indicate that gastric muscle relaxation plays an important role in the gastric protective mechanisms of SHXT, and the endogenous prostaglandins and the sulfhydryl compounds are not necessary for the action of SHXT.
我们研究了传统中药三黄泻心汤（SHXT）对胃黏膜保护作用的机制。十二指肠内给予SHXT可抑制幽门结扎大鼠的胃酸分泌。在给予阿司匹林、盐酸阿司匹林和乙醇前30分钟口服SHXT，可保护胃黏膜免受这些药物诱导的胃损伤，尽管它对吲哚美辛和水浸应激诱导的胃损伤没有影响。口服SHXT后30分钟测量时，胃内容物增加（肌肉松弛）。SHXT在体外也能抑制卡巴胆碱收缩的胃肌。在SHXT给药4小时后进行测试时，未观察到胃内容物增加或SHXT对乙醇损伤的抑制作用。用前列腺素合成抑制剂吲哚美辛和巯基阻断剂N-乙基马来酰亚胺预处理，既不影响SHXT引起的胃内容物增加，也不影响其对乙醇损伤的抑制作用。这些结果表明，胃肌松弛在SHXT的胃保护机制中起重要作用，内源性前列腺素和巯基化合物对SHXT的作用不是必需的。
