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嗜热古菌运动脱硫球菌中延伸因子EF-1α和EF-2以及核糖体蛋白S7和S10的基因的染色体组织和核苷酸序列。

Chromosomal organization and nucleotide sequence of the genes for elongation factors EF-1 alpha and EF-2 and ribosomal proteins S7 and S10 of the hyperthermophilic archaeum Desulfurococcus mobilis.

作者信息

Ceccarelli E, Bocchetta M, Creti R, Sanangelantoni A M, Tiboni O, Cammarano P

机构信息

Instituto Pasteur Fondazione Cenci-Bolognetti, Dipartimento di Biopatologia Umana, Università di Roma I, Policlinico Umberto I, Italy.

出版信息

Mol Gen Genet. 1995 Mar 20;246(6):687-96. doi: 10.1007/BF00290714.

DOI:10.1007/BF00290714
PMID:7898436
Abstract

The Desulfurococcus mobilis genes fus (encoding EF-2) and tuf (for EF-1 alpha) were cloned and sequenced together with genes for ribosomal proteins S10 (rps10) and S7 (rps7). Unlike Methanococcus, which displays the bacterial-like fus and tuf gene context 5'-rps12-rps7-fus-tuf-3', and similar to Sulfolobus and Pyrococcus, the Desulfurococcus fus gene (734 codons) has a distinct chromosomal location. Moreover, tuf (441 codons) is the promoter-proximal unit of a three-gene cluster comprising the genes rps10 (98 codons) and tRNA(Ser); the arrangement of the cluster is 5'-tuf-91 bp spacer -rps10-138 bp spacer -tRNA(Ser)-3' and the tuf gene is preceded by a canonical archaeal promoter. The D. mobilis gene rps7 (198 codons) is located further upstream from tuf (535 bp 'silent' intergenic spacing) and no rps12 homolog occurs in its immediate vicinity. Also, judging from putative promoter and transcription termination sequences, rps7 appears to be separately transcribed. Analysis of the predicted fus and tuf gene products revealed the three consensus motifs characteristic of GTP-binding proteins, and the fus-encoded EF-2 protein also displayed the consensus sequence required for ADP-ribosylation by Diphtheria toxin. Both EF sequences were definitely crenarchaeal by comparison with available homologs from other Archaea. Outgroup-rooted phylogenies derived from the sequences of ribosomal proteins S10 and S7 yielded the Sulfolobus-Desulfurococcus association at a high bootstrap confidence level.

摘要

将运动脱硫球菌（Desulfurococcus mobilis）的fus基因（编码延伸因子2，EF-2）和tuf基因（编码延伸因子1α，EF-1α）与核糖体蛋白S10（rps10）和S7（rps7）的基因一起进行了克隆和测序。与呈现细菌样fus和tuf基因排列顺序5'-rps12-rps7-fus-tuf-3'的甲烷球菌不同，脱硫球菌的fus基因（734个密码子）具有独特的染色体定位，类似于硫化叶菌属（Sulfolobus）和嗜热栖热菌属（Pyrococcus）。此外，tuf基因（441个密码子）是一个三基因簇中靠近启动子的单元，该三基因簇包含rps10基因（98个密码子）和tRNA(Ser)；该簇的排列顺序为5'-tuf-91 bp间隔区-rps10-138 bp间隔区-tRNA(Ser)-3'，且tuf基因之前有一个典型的古菌启动子。运动脱硫球菌的rps7基因（198个密码子）位于tuf基因的更上游（基因间隔区为535 bp的“沉默”间隔），且在其紧邻区域没有rps12同源物。同样，从推测的启动子和转录终止序列判断，rps7似乎是单独转录的。对预测的fus和tuf基因产物的分析揭示了GTP结合蛋白特有的三个共有基序，并且fus编码的EF-2蛋白还显示出被白喉毒素进行ADP核糖基化所需的共有序列。与其他古菌的可用同源物相比，两个EF序列肯定属于泉古菌门。从核糖体蛋白S10和S7的序列得出的外群生根系统发育树在高自展置信水平下显示出硫化叶菌属 - 脱硫球菌属的关联。

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本文引用的文献

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Novel expression of the ribosomal RNA genes in the extreme thermophile and archaebacterium Desulfurococcus mobilis.极端嗜热古菌脱硫球菌核糖体 RNA 基因的新型表达。
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