Maeda K, Yasuda M, Kaneda H, Maeda S, Yamadori A
Hyogo Institute for Aging Brain and Cognitive Disorders, Hyogo Brain and Heart Center at Himeji, Japan.
Neuropeptides. 1994 Dec;27(6):323-32. doi: 10.1016/0143-4179(94)90058-2.
We have measured cerebrospinal fluid (CSF) neuropeptide Y-like immunoreactivity (NPY-LI) and somatostatin-like immunoreactivity (SLI) in control subjects and in patients with various neurologic disorders. We observed a significant reduction in CSF SLI in control subjects over 60 years of age, compared with the younger controls. CSF SLI was significantly decreased in multiple sclerosis (MS), or Guillain-Barre syndrome, compared with that of age-matched control subjects. A reduced concentration of NPY-LI was found in CSF of patients with MS. We have also examined the molecular heterogeneity of peptide-LI in CSF. Gel chromatography, not high performance liquid chromatography (HPLC), suggested two NPY immunoreactive materials in CSF. Gel chromatography and HPLC revealed three SLI components in CSF: somatostatin 14, somatostatin 28 and a higher molecular weight precursor. Our results suggest that 1) there may be more than one form of NPY in human CSF, and 2) somatostatin neurons might be more susceptible to alteration than NPY neurons in various pathological conditions and aging.
我们测定了对照组以及患有各种神经系统疾病患者的脑脊液（CSF）中神经肽Y样免疫反应性（NPY-LI）和生长抑素样免疫反应性（SLI）。我们观察到，与年轻对照组相比，60岁以上对照组的脑脊液SLI显著降低。与年龄匹配的对照组相比，多发性硬化症（MS）或格林-巴利综合征患者的脑脊液SLI显著降低。在MS患者的脑脊液中发现NPY-LI浓度降低。我们还研究了脑脊液中肽-LI的分子异质性。凝胶色谱法而非高效液相色谱法（HPLC）表明脑脊液中有两种NPY免疫反应性物质。凝胶色谱法和HPLC显示脑脊液中有三种SLI成分：生长抑素14、生长抑素28和一种分子量更高的前体。我们的结果表明：1）人脑脊液中可能存在不止一种形式的NPY；2）在各种病理状况和衰老过程中，生长抑素神经元可能比NPY神经元更容易发生改变。
