Kilpatrick T J, Cheema S S, Koblar S A, Tan S S, Bartlett P F
Walter and Eliza Hall Institute, Parkville, Melbourne, Australia.
Neurosci Lett. 1994 Nov 7;181(1-2):129-33. doi: 10.1016/0304-3940(94)90576-2.
Primary neuroepithelial precursor cells carrying the reporter gene lacZ were transplanted into postnatal murine brain and assessed for their engraftment capacity. Freshly dissected precursors, derived from lacZ transgenic embryonic day 10 mouse brain, predominantly engrafted as discrete clusters, whereas the same precursors cultured in vitro with fibroblast growth factor-2, engrafted as single cells within the parenchyma of the hippocampus. Approximately 0.5% of the transplanted cells survived in the host brain for up to 3 months. Many of these cells displayed neuronal and astrocyte morphologies. These observations suggest that transplanted primary precursors derived from the embryonic brain can engraft and commit in situ to a variety of developmental fates.
携带报告基因lacZ的原代神经上皮前体细胞被移植到出生后的小鼠大脑中，并评估它们的植入能力。从lacZ转基因胚胎第10天小鼠大脑中新鲜分离的前体细胞，主要以离散的簇状形式植入，而在体外与成纤维细胞生长因子-2一起培养的相同前体细胞，则以单个细胞的形式植入海马实质内。大约0.5%的移植细胞在宿主大脑中存活长达3个月。这些细胞中的许多呈现出神经元和星形胶质细胞的形态。这些观察结果表明，源自胚胎大脑的移植原代前体细胞可以在原位植入并分化为多种发育命运。
