Richards L J, Murphy M, Dutton R, Kilpatrick T J, Puche A C, Key B, Tan S S, Talman P S, Bartlett P F
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10079-83. doi: 10.1073/pnas.92.22.10079.
We have investigated the differentiation potential of precursor cells within the developing spinal cord of mice and have shown that spinal cord cells from embryonic day 10 specifically give rise to neurons when plated onto an astrocytic monolayer, Ast-1. These neurons had the morphology of motor neurons and > 83% expressed the motor neuron markers choline acetyltransferase, peripherin, calcitonin gene-related peptide, and L-14. By comparison, < 10% of the neurons arising on monolayers of other neural cell lines or 3T3 fibroblasts had motor neuron characteristics. Cells derived from dorsal, intermediate, and ventral regions of the spinal cord all behaved similarly and gave rise to motor neuron-like cells when plated onto Ast-1. By using cells that expressed the lacZ reporter gene, it was shown that > 93% of cells present on the Ast-1 monolayers were motor neuron-like. Time-lapse analysis revealed that the precursors on the Ast-1 monolayers gave rise to neurons either directly or following a single cell division. Together, these results indicate that precursors in the murine spinal cord can be induced to differentiate into the motor neuron phenotype by factors produced by Ast-1 cells, suggesting that a similar factor(s) produced by cells akin to Ast-1 may regulate motor neuron differentiation in vivo.
我们研究了小鼠发育中的脊髓内前体细胞的分化潜能，并表明来自胚胎第10天的脊髓细胞在接种到星形胶质细胞单层Ast-1上时会特异性地分化为神经元。这些神经元具有运动神经元的形态，并且超过83%表达运动神经元标志物胆碱乙酰转移酶、外周蛋白、降钙素基因相关肽和L-14。相比之下，在其他神经细胞系或3T3成纤维细胞单层上产生的神经元中，只有不到10%具有运动神经元特征。来自脊髓背侧、中间和腹侧区域的细胞表现相似，接种到Ast-1上时会产生运动神经元样细胞。通过使用表达lacZ报告基因的细胞，发现Ast-1单层上超过93%的细胞是运动神经元样的。延时分析显示，Ast-1单层上的前体细胞要么直接分化为神经元，要么在经历一次细胞分裂后分化为神经元。这些结果共同表明，小鼠脊髓中的前体细胞可以被Ast-1细胞产生的因子诱导分化为运动神经元表型，这表明类似于Ast-1的细胞产生的类似因子可能在体内调节运动神经元的分化。
