Uchida T, Wada C, Wang C, Ishida H, Egawa S, Yokoyama E, Ohtani H, Koshiba K
Department of Urology, Kitasato University School of Medicine, Kanagawa, Japan.
Oncogene. 1995 Mar 2;10(5):1019-22.
Assessment of the genetic instability of a microsatellite has indicated a new mechanism in human carcinogenesis. Examination was made to determine whether microsatellite instability is associated with the onset of prostate cancer. Twenty-nine DNA samples from 24 primary prostate cancer, two metastatic lymph-node and three benign prostatic hypertrophy patients were used. Differences in unrelated microsatellites for tumor and normal DNA were detected in nine of 24 (37.5%) cases. Seven of 11 (63.6%) with poorly differentiated adenocarcinomas and seven of 15 (46.7%) stage D metastatic patients showed somatic instability in a number of microsatellites. Statistically significant differences in well to moderately differentiated tumors and poorly differentiated cancer (P = 0.015, Chi-square test), were detected but not for stages A-C and D (P = 0.2311). Genetic alterations would thus appear to be rare in low grade and/or early prostate cancers but more common in high grade and/or advanced prostate cancers.
对微卫星基因不稳定性的评估揭示了人类致癌作用中的一种新机制。开展相关检查以确定微卫星不稳定性是否与前列腺癌的发病有关。使用了来自24例原发性前列腺癌、2例转移性淋巴结以及3例良性前列腺增生患者的29份DNA样本。在24例（37.5%）病例中的9例检测到肿瘤DNA和正常DNA在不相关微卫星上存在差异。11例低分化腺癌患者中的7例（63.6%）以及15例D期转移性患者中的7例（46.7%）在多个微卫星上表现出体细胞不稳定性。在高分化至中分化肿瘤与低分化癌之间检测到具有统计学意义的差异（P = 0.015，卡方检验），但在A - C期和D期之间未检测到差异（P = 0.2311）。因此，基因改变在低级别和/或早期前列腺癌中似乎很少见，但在高级别和/或晚期前列腺癌中更常见。
