Kaneko Y, Nonoguchi K, Fukuyama H, Takano S, Higashitsuji H, Nishiyama H, Takenawa J, Nakayama H, Fujita J
Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Japan.
Oncogene. 1995 Mar 2;10(5):945-52.
From a mouse brain cDNA library, two species of the Csk-type tyrosine kinase (ctk) gene containing different 5' untranslated sequences were cloned. Using the common as well as specific nucleotide sequences of the two clones as probes, we examined the expression of ctk in various mouse tissues by Northern blot analysis. The results indicated that both species of ctk were expressed in the brain, testis and bone marrow. By in situ histochemistry of the brain, ctk transcript was detected in neurons throughout the entire brain, especially those of the cortex, the hippocampus and the cerebellum. This distribution pattern is similar to that of the Src family kinases including Yes, Src, Fyn and Lyn. In the testis, the major transcript (0.7 kb) was shorter than that expressed in the brain and the bone marrow (2.0 kb). A subsequent Northern blot analysis of fractionated germ cell populations and in situ histochemistry revealed that the short and long transcripts were expressed in germ cells and somatic cells, respectively, and that the expression level was quantitatively regulated during germ cell development. These results suggest that Ctk is involved in the regulation of neural function and differentiation of male germ cells through interactions with member(s) of the Src family kinases.
从小鼠脑cDNA文库中克隆出两种含有不同5'非翻译序列的Csk型酪氨酸激酶（ctk）基因。以这两个克隆的共同及特异核苷酸序列作为探针，我们通过Northern印迹分析检测了ctk在小鼠各种组织中的表达。结果表明，两种ctk在脑、睾丸和骨髓中均有表达。通过脑的原位组织化学分析，在整个脑中的神经元，尤其是皮质、海马和小脑中的神经元中检测到ctk转录本。这种分布模式与Src家族激酶（包括Yes、Src、Fyn和Lyn）的分布模式相似。在睾丸中，主要转录本（0.7 kb）比在脑和骨髓中表达的转录本（2.0 kb）短。随后对分离的生殖细胞群体进行的Northern印迹分析和原位组织化学显示，短转录本和长转录本分别在生殖细胞和体细胞中表达，并且在生殖细胞发育过程中表达水平受到定量调节。这些结果表明，Ctk通过与Src家族激酶成员相互作用参与神经功能调节和雄性生殖细胞分化。
