Besset V, Rhee K, Wolgemuth D J
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Mol Reprod Dev. 1998 May;50(1):18-29. doi: 10.1002/(SICI)1098-2795(199805)50:1<18::AID-MRD3>3.0.CO;2-#.
We have isolated a murine cDNA encoding for a novel putative Cdk-related protein kinase, which has been named Pftaire-1, by screening a testis cDNA library for new serine/threonine kinases. Pftaire-1 showed 50% and 49% amino acid identity with Cdk5 and Pctaire-3, respectively, and contains the eleven subdomains characteristic of the protein kinases. By northern blot analysis we detected two transcripts of approximately 5.5 and 4.9 kb in size. These transcripts were expressed at low level in all murine tissues tested, except in the brain, testis and embryo, where high expression was detected. Cellular localization of the mRNAs by in situ hybridization analysis shows that Pftaire-1 is expressed in late pachytene spermatocytes in the testis and in post mitotic neuronal cells both in the brain and the embryo, suggesting a role of Pftaire-1 both in the process of meiosis as well as neuron differentiation and/or function.
我们通过筛选睾丸cDNA文库寻找新的丝氨酸/苏氨酸激酶，分离出了一个编码新型假定Cdk相关蛋白激酶的小鼠cDNA，该激酶被命名为Pftaire-1。Pftaire-1与Cdk5和Pctaire-3的氨基酸同一性分别为50%和49%，并含有蛋白激酶特有的11个亚结构域。通过Northern印迹分析，我们检测到两条大小约为5.5 kb和4.9 kb的转录本。除了在大脑、睾丸和胚胎中检测到高表达外，这些转录本在所有测试的小鼠组织中均低水平表达。通过原位杂交分析对mRNA进行细胞定位表明，Pftaire-1在睾丸中的晚粗线期精母细胞以及大脑和胚胎中的有丝分裂后神经元细胞中表达，提示Pftaire-1在减数分裂过程以及神经元分化和/或功能中均发挥作用。
