用于唐氏综合征产前筛查的流式细胞术检测血液中性粒细胞尿素抗性碱性磷酸酶的方法。
Flow cytometric method to measure urea-resistant alkaline phosphatase from blood neutrophils for use in the prenatal screening of Down's syndrome.
作者信息
Agrawal Y P, Ylikangas P, Parviainen M T, Penttilä I M
机构信息
Department of Clinical Chemistry, Kuopio University Hospital, Finland.
出版信息
Prenat Diagn. 1994 Dec;14(12):1141-9. doi: 10.1002/pd.1970141207.
The histochemical measurement of urea-resistant alkaline phosphatase from maternal blood neutrophils is known to have a high detection rate for the prenatal detection of Down's syndrome pregnancies. However, because the histochemical method is laborious and subjective to use, it has not gained widespread acceptance in prenatal screening programmes. We present a simple and objective method for the measurement of urea-resistant alkaline phosphatase by flow cytometry. The method should allow the design of larger studies aimed at evaluating the role of neutrophil urea-resistant alkaline phosphatase in the prenatal screening for Down's syndrome.
已知通过对母血中性粒细胞进行耐尿素碱性磷酸酶的组织化学测量，对唐氏综合征妊娠的产前检测具有较高的检出率。然而，由于组织化学方法使用起来费力且主观，它在产前筛查项目中并未得到广泛认可。我们提出了一种通过流式细胞术测量耐尿素碱性磷酸酶的简单且客观的方法。该方法应有助于设计更大规模的研究，旨在评估中性粒细胞耐尿素碱性磷酸酶在唐氏综合征产前筛查中的作用。
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