四氢苯并补骨脂素与DNA光结合的序列特异性

Sequence specificity of tetrahydrobenzopsoralen photobinding to DNA.

作者信息

Mobilio S, Tondelli L, Capobianco M, Gia O

机构信息

Department of Pharmaceutical Sciences, Padua University, Italy.

出版信息

Photochem Photobiol. 1995 Feb;61(2):113-7. doi: 10.1111/j.1751-1097.1995.tb03948.x.

DOI:10.1111/j.1751-1097.1995.tb03948.x

PMID:7899500

Abstract

The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity was greatly dependent upon base composition. The two tetracyclic compounds show similar behavior in comparison to other bifunctional derivatives. Their overall rate constants were greatly enhanced in comparison to classical psoralens. However, their high efficiency of covalent binding is counterbalanced by low affinity for noncovalent interaction with DNA.

摘要

通过测试对多种自互补寡核苷酸的光反应性，研究了两种四氢苯并补骨脂素衍生物与DNA光结合的序列特异性。评估了与每个DNA序列非共价结合的热力学常数。光反应程度在很大程度上取决于碱基组成。与其他双功能衍生物相比，这两种四环化合物表现出相似的行为。与经典补骨脂素相比，它们的总速率常数大大提高。然而，它们共价结合的高效率被与DNA非共价相互作用的低亲和力所抵消。