Takafuta T, Fujimura K, Kawano H, Noda M, Fujimoto T, Oda K, Shimomura T, Kuramoto A
Department of Internal Medicine, Hiroshima University, Japan.
Thromb Haemost. 1994 Nov;72(5):762-9.
Glycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myristate-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.
糖蛋白V（GPV）是一种分子量为82kD的血小板膜蛋白，属于富含亮氨酸糖蛋白（LRG）之一。通过逆转录-聚合酶链反应（RT-PCR），从血小板和巨核细胞系的mRNA中扩增出GPV cDNA。然而，由于很少有报道表明GPV蛋白作为一种谱系和成熟特异性蛋白在巨核细胞中表达，我们使用一种识别GPV的新型单克隆抗体（1D9）在蛋白质水平上研究了GPV的表达。流式细胞术和免疫组织化学分析表明，仅在骨髓穿刺物中的巨核细胞表面和细胞质中检测到GPV。在巨核细胞系UT-7中，用佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）处理后，GPV抗原增加。这些数据表明，在造血细胞中只有巨核细胞特异性表达GPV蛋白，并且GPV的表达随着巨核细胞向GPIb-IX复合物的分化而增加。
