Aiello F B, Gusella L, Longo D L, Birchenall-Roberts M, Takacs L, Takei F, Ruscetti F, Musiani P, Durum S K
Biological Carcinogenesis and Development Program, Program Resources Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD.
Immunopharmacol Immunotoxicol. 1993 Aug;15(4):327-53. doi: 10.3109/08923979309035232.
Costimulatory molecules in addition to occupancy of the T-cell antigen receptor, are required to induce T-cell proliferation. Previous work suggested that membrane molecules responsible for costimulatory activity were not constitutively expressed on the antigen presenting cell (APC) surface. In the present study, we have identified a cloned macrophage cell line (FLJ2) with inducible APC function. The unactivated FLJ2 line could not induce T-cell proliferation. FLJ2 could present alloantigen, and stimulate proliferation of either a T-cell clone or normal resting T cells following activation with IFN gamma or unexpectedly with lipopolysaccharide (LPS)-Activated FLJ2 cells could be fixed and APC function was preserved. The relevant inducible molecules required for APC function appeared distinct from Ia and IL1. The expression of ICAM-1 and LFA-1 was increased during activation and anti-LFA-1 antibody blocked APC function. This suggests that one important feature of the activation process may be improvement of cellular adhesion.
除了T细胞抗原受体的占据外，共刺激分子对于诱导T细胞增殖也是必需的。先前的研究表明，负责共刺激活性的膜分子在抗原呈递细胞（APC）表面不是组成性表达的。在本研究中，我们鉴定了一种具有可诱导APC功能的克隆巨噬细胞系（FLJ2）。未活化的FLJ2细胞系不能诱导T细胞增殖。FLJ2可以呈递同种异体抗原，并在经γ干扰素激活后，或者意外地经脂多糖（LPS）激活后，刺激T细胞克隆或正常静息T细胞的增殖。活化的FLJ2细胞可以固定，并且APC功能得以保留。APC功能所需的相关可诱导分子似乎与Ia和IL-1不同。ICAM-1和LFA-1的表达在激活过程中增加，抗LFA-1抗体阻断了APC功能。这表明激活过程的一个重要特征可能是细胞黏附的改善。
