Vernazza P L, Eron J J, Fiscus S A
Dept. of Medicine, University of North Carolina at Chapel Hill 27599-7030.
J Infect Dis. 1993 Nov;168(5):1269-72. doi: 10.1093/infdis/168.5.1269.
Quantitation of human immunodeficiency virus type 1 (HIV-1) in plasma is frequently used to monitor virus load and assess the effect of antiviral agents, but information regarding the stability of the virus is limited. Plasma from patients with CD4 cell counts of < 300/microL was tested by quantitative cultures 1, 4, and 24 h after phlebotomy and after storage at -75 degrees C. Viremia was detected in 18 (69%) of 26 patients. Of 16 samples, 2 (13%) at 4 h and 6 (38%) at 24 h had a significantly lower titer than the sample cultured at 1 h. No culture result after the freezing step was significantly different from the 1-h reference value. The decrease in virus titer over time was observed primarily in patients with CD4 cell counts of > 100/microL. Plasma should not be stored for > 2-4 h at room temperature for HIV-1 culture. If immediate processing is not an option, plasma can be frozen shortly after phlebotomy for later testing without a significant loss of infectious virus.
对血浆中1型人类免疫缺陷病毒（HIV-1）进行定量检测常用于监测病毒载量并评估抗病毒药物的疗效，但关于该病毒稳定性的信息有限。对CD4细胞计数低于300/微升的患者的血浆，在采血后1小时、4小时和24小时以及在-75℃储存后进行定量培养检测。26例患者中有18例（69%）检测到病毒血症。在16份样本中，4小时时2份（13%）、24小时时6份（38%）的滴度显著低于1小时培养的样本。冷冻步骤后的培养结果与1小时的参考值均无显著差异。病毒滴度随时间的下降主要在CD4细胞计数大于100/微升的患者中观察到。用于HIV-1培养的血浆在室温下储存不应超过2 - 4小时。如果无法立即处理，血浆可在采血后不久冷冻以便日后检测，而不会导致感染性病毒显著损失。
