Holodniy M, Mole L, Yen-Lieberman B, Margolis D, Starkey C, Carroll R, Spahlinger T, Todd J, Jackson J B
AIDS Research Center, Veterans Affairs Medical Center, Palo Alto, CA 94304, USA.
J Clin Microbiol. 1995 Jun;33(6):1562-6. doi: 10.1128/jcm.33.6.1562-1566.1995.
This study compared the levels of human immunodeficiency virus (HIV) virion RNA in plasma from whole blood collected in VACUTAINER CPT (cell preparation tube), VACUTAINER PPT (plasma preparation tube), VACUTAINER SST (serum separation tube), and standard VACUTAINER tubes with sodium heparin, acid citrate dextrose, sodium citrate, and potassium EDTA used as anticoagulants. Quantitative plasma HIV RNA levels were measured by branched-DNA signal amplification. Blood from all tubes was either processed within 1 to 3 h after collection or stored at room temperature or at 4 degrees C for analysis at 6 to 8 and 30 h postdraw. Immediately separated plasma from sodium citrate CPT tubes held at 4 degrees C maintained better stability of HIV RNA equivalents than whole blood held at room temperature or 4 degrees C. The highest number of HIV RNA equivalents was seen with EDTA VACUTAINER tubes. HIV RNA equivalents in all types of plasma were significantly higher than in SST tubes. Although a decline in HIV RNA equivalents was seen in all collection devices after 30 h, a significantly greater decline in plasma HIV RNA equivalents occurred in acid citrate dextrose VACUTAINER tubes than in citrate CPT, PPT, and standard EDTA VACUTAINER tubes. In order to minimize the variability of quantitative HIV RNA test results, our data suggest that samples collected for a particular assay should be processed at the same time postdraw using a particular tube type throughout a given study.
本研究比较了在VACUTAINER CPT（细胞制备管）、VACUTAINER PPT（血浆制备管）、VACUTAINER SST（血清分离管）以及使用肝素钠、枸橼酸葡萄糖、枸橼酸钠和乙二胺四乙酸钾作为抗凝剂的标准VACUTAINER管中采集的全血血浆中人类免疫缺陷病毒（HIV）病毒粒子RNA的水平。通过分支DNA信号扩增测量血浆HIV RNA定量水平。所有管中的血液在采集后1至3小时内进行处理，或在室温或4℃下储存，以便在采血后6至8小时和30小时进行分析。在4℃下保存的枸橼酸钠CPT管中立即分离出的血浆，其HIV RNA当量的稳定性优于在室温或4℃下保存的全血。使用乙二胺四乙酸VACUTAINER管时，HIV RNA当量数量最高。所有类型血浆中的HIV RNA当量均显著高于SST管中的。尽管30小时后所有采集装置中的HIV RNA当量均有所下降，但枸橼酸葡萄糖VACUTAINER管中血浆HIV RNA当量的下降幅度显著大于枸橼酸盐CPT管、PPT管和标准乙二胺四乙酸VACUTAINER管。为了尽量减少HIV RNA定量检测结果的变异性，我们的数据表明，在特定研究中，为特定检测采集的样本应在采血后同一时间使用特定管型进行处理。
