Rea M A, Buckley B, Lutton L M
Circadian Neurobiology Research Group, Armstrong Laboratory (AL/CFTO), Brooks Air Force Base, Texas 78235.
Am J Physiol. 1993 Nov;265(5 Pt 2):R1191-8. doi: 10.1152/ajpregu.1993.265.5.R1191.
Syrian hamsters were fitted with guide cannulas stereotaxically aimed at the suprachiasmatic nuclei (SCN) and housed in constant darkness in cages equipped with running wheels. Animals received 300-nl injections of either vehicle, CNQX, or MK-801 into the region of the SCN 5 min before a brief (10 min at 20 lx) light exposure. Local administration of either 1 mM CNQX or 1 mM MK-801 at circadian time (CT) 18 significantly inhibited light-induced phase advances (vehicle = 52 +/- 9 min; CNQX = 12 +/- 7 min; MK-801 = 12 +/- 5 min; P < 0.05 relative to vehicle+light group). The effects of both drugs were reversible and dose related. Injection of 1 mM MK-801 at CT13.5 resulted in a 71% inhibition of light-induced phase delays (vehicle = -51 +/- 6 min; MK-801 = -15 +/- 5 min; P < 0.05), while CNQX failed to significantly inhibit light-induced phase delays (-39 +/- 10 min). Local administration of either 1 mM CNQX or MK-801 into the SCN region reduced the number of Fos-immunoreactive cells relative to vehicle-injected controls by approximately 32 and 44%, respectively (vehicle = 951 +/- 79; CNQX = 643 +/- 135, P < 0.05; MK-801 = 533 +/- 143, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
叙利亚仓鼠被立体定位安装导向套管，套管对准视交叉上核（SCN），并饲养在配备有跑轮的持续黑暗的笼子里。在短暂（20勒克斯下10分钟）光照前5分钟，给动物在SCN区域注射300纳升的溶剂、CNQX或MK - 801。在昼夜节律时间（CT）18时，局部注射1毫摩尔/升的CNQX或1毫摩尔/升的MK - 801显著抑制了光诱导的相位提前（溶剂组 = 52±9分钟；CNQX组 = 12±7分钟；MK - 801组 = 12±5分钟；相对于溶剂+光照组，P < 0.05）。两种药物的作用都是可逆的且与剂量相关。在CT13.5注射1毫摩尔/升的MK - 801导致光诱导的相位延迟受到71%的抑制（溶剂组 = -51±6分钟；MK - 801组 = -15±5分钟；P < 0.05），而CNQX未能显著抑制光诱导的相位延迟（-39±10分钟）。相对于注射溶剂的对照组，在SCN区域局部注射1毫摩尔/升的CNQX或MK - 801分别使Fos免疫反应性细胞数量减少了约32%和44%（溶剂组 = 951±79；CNQX组 = 643±135，P < 0.05；MK - 801组 = 533±143，P < 0.05）。（摘要截短于250字）
