Bicudo M H
Department of Food Science and Nutrition, University of Minnesota, St. Paul.
Arq Gastroenterol. 1993 Jan-Mar;30(1):21-6.
This study was performed to evaluate the effect of lactose induced diarrhea on the key enzymes of glutamine metabolism in skeletal muscle and small intestine, in rats. As compared to weight paired controls, animals with diarrhea presented higher muscle glutamine synthetase activity associated with reduced skeletal muscle glutamine concentration with a fall in arterial glutamine and an increased intestinal glutaminase activity. These alterations are similar to those reported by others in conditions in which accelerated muscle proteolysis is likely to occur such as in sepsis and after surgery. Besides the data suggestive of an overall alterations in glutamine metabolism, an important finding of this study was the increase in specific activity of intestinal phosphate dependent glutaminase in rats with diarrhea. This enzyme has been shown not to respond to many conditions such as acidosis, alkalosis or increased glutamine ingestion through drinking water or diet.
本研究旨在评估乳糖诱导的腹泻对大鼠骨骼肌和小肠中谷氨酰胺代谢关键酶的影响。与体重配对的对照组相比，腹泻动物的肌肉谷氨酰胺合成酶活性较高，同时骨骼肌谷氨酰胺浓度降低，动脉谷氨酰胺水平下降，肠道谷氨酰胺酶活性增加。这些改变与其他人在可能发生加速肌肉蛋白水解的情况下（如脓毒症和手术后）所报道的相似。除了提示谷氨酰胺代谢总体改变的数据外，本研究的一个重要发现是腹泻大鼠肠道磷酸依赖性谷氨酰胺酶的比活性增加。已表明该酶对许多情况无反应，如酸中毒、碱中毒或通过饮水或饮食摄入增加谷氨酰胺。
