一个通用的靶序列在体外被多种同源结构域所结合。
A universal target sequence is bound in vitro by diverse homeodomains.
作者信息
Kalionis B, O'Farrell P H
机构信息
Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448.
出版信息
Mech Dev. 1993 Sep;43(1):57-70. doi: 10.1016/0925-4773(93)90023-q.
Abstract
To determine the number of DNA binding proteins capable of binding a consensus Engrailed binding site, this consensus sequence was used to screen a library of Drosophila cDNA clones in a bacteriophage expression vector. We retrieved clones encoding 20 distinct DNA binding domains, 17 of which are homeodomains. Binding to a variety of oligonucleotides confirms the related sequence specificity of the retrieved binding domains. Nonetheless, the homeodomains have remarkably diverse amino acid sequences. We conclude that during the evolutionary divergence of homeodomains, the specificity of DNA binding has been much more highly conserved than the amino acid sequence.
摘要
为了确定能够结合共有Engrailed结合位点的DNA结合蛋白的数量，该共有序列被用于筛选噬菌体表达载体中的果蝇cDNA克隆文库。我们获得了编码20个不同DNA结合结构域的克隆，其中17个是同源结构域。与多种寡核苷酸的结合证实了所获得的结合结构域具有相关的序列特异性。尽管如此，同源结构域的氨基酸序列却有显著差异。我们得出结论，在同源结构域的进化分歧过程中，DNA结合的特异性比氨基酸序列的保守程度要高得多。
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本文引用的文献
1
Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila.控制发育的基因间的结构关系：果蝇触角足、超双胸和腹沟基因座之间的序列同源性
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115-9. doi: 10.1073/pnas.81.13.4115.
2
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
3
Molecular cloning and chromosome mapping of a mouse DNA sequence homologous to homeotic genes of Drosophila.与果蝇同源异型基因同源的小鼠DNA序列的分子克隆及染色体定位
Cell. 1984 Oct;38(3):675-80. doi: 10.1016/0092-8674(84)90262-9.
4
The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity.果蝇发育基因“engrailed”编码一种序列特异性DNA结合活性。
Nature. 1985;318(6047):630-5. doi: 10.1038/318630a0.
5
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Genetics. 1985 May;110(1):17-72. doi: 10.1093/genetics/110.1.17.
6
Sequence analysis and neuronal expression of fasciclin I in grasshopper and Drosophila.蝗虫和果蝇中 Fasciclin I 的序列分析及神经元表达
Cell. 1988 May 20;53(4):577-87. doi: 10.1016/0092-8674(88)90574-0.
7
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9
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10
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Cell. 1988 Sep 23;54(7):1081-90. doi: 10.1016/0092-8674(88)90123-7.
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