Bradford G, Williams N, Barber L, Bertoncello I
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Exp Hematol. 1993 Dec;21(13):1615-20.
Delayed platelet recovery and altered marrow megakaryocytopoiesis was observed in highly irradiated mice following transplantation with 1000 lineage-negative, stem cell antigen-positive (Lin-Sca-1+) cells. Thirty days after transplantation, the mice were thrombocytopenic. Normal platelet levels were reestablished by 90 days after transplantation. Platelet levels were established from the bone marrow with altered megakaryocytopoiesis, however. Megakaryocyte progenitor numbers were found at reduced levels in the reconstituted marrow at all time points assessed. Bone marrow function was also different in that marrow reserve was also diminished. While the transplantation regime did give long-term reconstitution of blood cells, the quality of the marrow reserve was significantly impaired as revealed by response to further hematopoietic challenge. The data indicate that mice transplanted with a population of highly defined stem cells have perturbed marrow function, one characteristic being an altered process of megakaryocytopoiesis.
在用1000个谱系阴性、干细胞抗原阳性（Lin-Sca-1+）细胞移植后的高辐射小鼠中，观察到血小板恢复延迟和骨髓巨核细胞生成改变。移植后30天，小鼠出现血小板减少。移植后90天血小板水平恢复正常。然而，血小板水平是由骨髓巨核细胞生成改变的骨髓建立的。在所有评估时间点，在重建的骨髓中发现巨核细胞祖细胞数量减少。骨髓功能也有所不同，因为骨髓储备也减少了。虽然移植方案确实能使血细胞长期重建，但对进一步造血挑战的反应表明，骨髓储备质量明显受损。数据表明，移植了高度明确的干细胞群体的小鼠骨髓功能受到干扰，一个特征是巨核细胞生成过程改变。
