Nibley W E, Pohlmann S J, Spangrude G J
Department of Medicine, University of Utah, Salt Lake City, USA.
Stem Cells. 1997;15 Suppl 1:31-8; discussion 38-9. doi: 10.1002/stem.5530150806.
The kinetics of blood and organ engraftment following transplants of defined populations of hematopoietic stem/progenitor cells were investigated utilizing cell populations defined by surface antigen and rhodamine-123 staining. While long-term repopulating stem cells, short-term multipotent progenitors and committed progenitors all reconstituted peripheral blood red cells and splenic cellularity, only the population of cells that includes highly enriched long-term repopulating stem cells (Thy-1.1lowLinnegSca-1+Rh123low) reconstituted marrow cellularity. In addition, peripheral blood platelet and nucleated cell count increased only after transplant of the long-term repopulating population. These results argue that the major cell population that functions to reconstitute hematopoiesis after bone marrow transplantation is a primitive, marrow-homing stem cell. Transplantation of highly enriched multipotent progenitors that lack long-term reconstituting potential had no impact on hematopoietic recovery, apart from a transient increase in circulating erythrocytes. These results suggest that the primary cell population that functions to reconstitute hematopoiesis in a transplant setting is the long-term repopulating stem cell. This observation is discussed in the context of the normal hematopoietic process.
利用通过表面抗原和罗丹明-123染色定义的细胞群体，研究了特定群体造血干/祖细胞移植后血液和器官植入的动力学。虽然长期重建造血干细胞、短期多能祖细胞和定向祖细胞都能重建外周血红细胞和脾细胞数量，但只有包含高度富集的长期重建造血干细胞（Thy-1.1lowLinnegSca-1+Rh123low）的细胞群体能重建骨髓细胞数量。此外，只有在移植长期重建造血群体后，外周血血小板和有核细胞计数才会增加。这些结果表明，骨髓移植后发挥造血重建功能的主要细胞群体是原始的、归巢至骨髓的干细胞。缺乏长期重建潜力的高度富集多能祖细胞移植，除了使循环红细胞短暂增加外，对造血恢复没有影响。这些结果表明，在移植环境中发挥造血重建功能的主要细胞群体是长期重建造血干细胞。在正常造血过程的背景下讨论了这一观察结果。
